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Invitrogen
Phospho-Tau (Thr212, Ser214) Monoclonal Antibody (AT100)
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FIGURE: 1 / 22
Phospho-Tau (Thr212, Ser214) Antibody (MN1060) in IHC (P)
Acta neuropathologica communications 2016 -
Fig. 6 Tau pathology in young and aged degus. Phosphoepitope-specific antibodies were used to examine involvement of tau. AT8 revealed intracellular immunoreactivity in young and aged degus ( a , b ). AT100 was unspecific and appeared almost entirely in the nucleus ( c , d ). AT180-labelled cortical neurons in young and aged animals ( e , f ). Scale bar = 100 mum
Product Details
MN1060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Chicken,
Fruit fly,
Hamster,
Human,
Mammal,
Mouse,
Non-human primate,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
AT100
Immunogen
Purified human Tau
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MN1060 recognized PHF-Tau. No cross reactivity with normal biopsy-or-autopsy-derived Tau or fetal Tau has been observed. This suggests that this antibody detects an abnormal phosphorylation site unique to PHF-Tau. The epitope of this antibody has been show to contain phosphorylated Ser214 and phosphorylated Thr212 residues (numbering according to human Tau40).
MN1060 detects PHF-tau (Thr212/Ser214) which has a predicted molecular weight of approximately 79 kDa.
This product is a Low Endotoxin formulation.
Purity is >95% as determined by SDS-PAGE.
Target Information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau-4; Tau-derived paired helical filament hexapeptide; Tau5; unnamed protein product
Gene Aliases: DDPAC; FTD1; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU; tau-40; Tau-PHF6
UniProt ID: (Human) P10636
Entrez Gene ID: (Human) 4137
DNA binding
AT DNA binding
double-stranded DNA binding
single-stranded DNA binding
RNA binding
actin binding
protein binding
microtubule binding
tubulin binding
SH3 domain binding
enzyme binding
protein kinase binding
protein binding, bridging
apolipoprotein binding
dynactin binding
phosphatidylinositol binding
identical protein binding
sequence-specific DNA binding
chaperone binding
protein phosphatase 2A binding
Hsp90 protein binding
lipoprotein particle binding
histone-dependent DNA binding
microtubule lateral binding
phosphatidylinositol bisphosphate binding
microtubule cytoskeleton organization
microglial cell activation
cellular response to DNA damage stimulus
cell-cell signaling
synapse assembly
learning or memory
memory
response to lead ion
regulation of autophagy
negative regulation of gene expression
negative regulation of mitochondrial membrane potential
rRNA metabolic process
axonal transport of mitochondrion
central nervous system neuron development
regulation of microtubule polymerization or depolymerization
regulation of microtubule polymerization
positive regulation of microtubule polymerization
cytoplasmic microtubule organization
neuron projection development
positive regulation of superoxide anion generation
regulation of chromosome organization
stress granule assembly
cellular response to heat
cellular response to reactive oxygen species
positive regulation of axon extension
microtubule polymerization
astrocyte activation
regulation of synaptic plasticity
intracellular distribution of mitochondria
generation of neurons
synapse organization
regulation of calcium-mediated signaling
protein polymerization
protein homooligomerization
axon development
regulation of microtubule cytoskeleton organization
plus-end-directed organelle transport along microtubule
regulation of mitochondrial fission
negative regulation of mitochondrial fission
fibril organization
axonal transport
regulation of cellular response to heat
regulation of long term synaptic depression
positive regulation of protein localization to synapse
neurofibrillary tangle assembly
negative regulation of establishment of protein localization to mitochondrion
positive regulation of cellular protein localization
negative regulation of tubulin deacetylation
amyloid fibril formation
cellular response to nerve growth factor stimulus
cellular response to brain-derived neurotrophic factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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