Invitrogen

MNS1 Polyclonal Antibody

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MNS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of MNS1 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MNS1 Polyclonal Antibody (Product # PA5-59016).

MNS1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MNS1 in human testis using a MNS1 Polyclonal Antibody (Product # PA5-59016) shows moderate positivity in seminiferous ducts.

MNS1 Antibody in Western Blot (WB) — Western blot analysis of MNS1 in control (vector only transfected HEK293T lysate) and MNS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a MNS1 Polyclonal Antibody (Product # PA5-59016).

Product Details

PA5-59016

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MNS1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2644024

Product Specific Information

Immunogen sequence: AKEEEENFRK TMLAKFAEDD RIELMNAQKQ RMKQLEHRRA VEKLIEERRQ QFLADKQREL EEWQLQQRRQ GFINAIIEEE RLKLLKEHAT NLLGYLPKGV FKKEDDIDLL GEEFRKVYQQ R

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 87%.

Target Information

MNS1 may play a role in the control of meiotic division and germ cell differentiation through regulation of pairing and recombination during meiosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

RNA sequencing and immunofluorescence of the myotendinous junction of mature horses and humans.

American journal of physiology. Cell physiology

Jakobsen JR,Schjerling P,Svensson RB,Buhl R,Carstensen H,Koch M,Krogsgaard MR,Kjær M,Mackey AL

PA5-59016 was used in Immunohistochemistry (Frozen) to conclude that, in muscle-tendon interface tissue enriched with myofiber tips, we identified the expression of previously unknown MTJ genes representing diverse biological processes, which may be important in the maintenance of the specialized MTJ.

Wed Sep 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: meiosis-specific nuclear structural 1; Meiosis-specific nuclear structural protein 1; spermatogenesis associated 40

Gene Aliases: MNS1; SPATA40

UniProt ID: (Human) Q8NEH6

Entrez Gene ID: (Human) 55329

Molecular Function: structural protein

Function(s)

spermatogenesis cilium organization positive regulation of cilium assembly meiotic cell cycle left/right axis specification

Process(es)

identical protein binding