Invitrogen

MPI Polyclonal Antibody

View all (30) MPI antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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MPI Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MPI in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with MPI polyclonal antibody (Product # PA5-95402) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

MPI Antibody in Western Blot (WB) — Western blot analysis of MPI in rat testis extract (lane 1) and HeLa whole cell lysates (lane 2). Samples were incubated with MPI polyclonal antibody (Product # PA5-95402) using a 0.5 µg/mL dilution. developed was performed using enhanced chemiluminescence (ECL).

Product Details

PA5-95402

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	0.5-1 µg/mL	-
Immunocytochemistry (ICC/IF)	0.5-1 µg/mL	-
Flow Cytometry (Flow)	1-3 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human MPI recombinant protein (Position: A2-K99). View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807205

Product Specific Information

Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mannose phosphate isomerase 1; Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI

Gene Aliases: 1110002E17Rik; AI315153; CDG1B; MPI; Mpi-1; Mpi1; PMI; PMI1

UniProt ID: (Human) Q96AB0, (Rat) Q68FX1, (Mouse) Q924M7

Entrez Gene ID: (Human) 4351, (Rat) 300741, (Mouse) 110119

Molecular Function: isomerase