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Invitrogen
MPP10 Polyclonal Antibody
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FIGURE: 1 / 1
MPP10 Antibody (PA5-87589) in WB
Western blot analysis of MPP10 in extracts of various cell lines. Samples were incubated with MPP10 Polyclonal antibody (Product # PA5-87589) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-87589
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 432-681 of human MPHOSPH10 (NP_0057821)
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: RIRDQAWDDV VRKEKPKEDA YEYKKRLTLD HEKSKLSLAE IYEQEYIKLN QQKTAEEENP EHVEIQKMMD SLFLKLDALS NFHFIPKPPV PEIKVVSNLP AITMEEVAPV SVSDAALLAP EEIKEKNKAG DIKTAAEKTA TDKKRERRKK KYQKRMKIKE KEKRRKLLEK SSVDQAGKYS KTVASEKLKQ LTKTGKASFI KDEGKDKALK SSQAFFSKLQ DQVKMQINDA KKTEKKKKKR QDISVHKLKL; Positive Samples: MCF-7, HeLa; Cellular Location: Chromosome, Nucleus, nucleolus
Target Information
MPP10 (M-phase phosphoprotein 10), also known as MPHOSPH10, is a 681 amino acid protein that localizes to the fibrillar region of the nucleolus. Phosphorylated in the M (mitotic) phase of the cell cycle, MPP10 is a component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex and is required for the early cleavages during pre-18S ribosomal RNA processing. MPP10 forms a heterotrimeric complex with IMP-3 and IMP-4, which may be required for its association with nucleolar components. The gene encoding MPP10 is localized to human chromosome 2, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: M phase phosphoprotein 10; M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein); protein phosphatase 1, regulatory subunit 106; U3 small nucleolar ribonucleoprotein; U3 small nucleolar ribonucleoprotein protein MPP10
Gene Aliases: 2810453H10Rik; 5730405D16Rik; AI326008; CT90; MPHOSPH10; MPP10; MPP10P; PPP1R106
UniProt ID: (Human) O00566, (Mouse) Q810V0
Entrez Gene ID: (Human) 10199, (Mouse) 67973
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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