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Bethyl Laboratories
MPP11 Polyclonal Antibody
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FIGURE: 1 / 1
MPP11 Antibody (A303-722A) in WB
Detection of human MPP11 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MPP11 antibody (Product # A303-722A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. MPP11 was also immunoprecipitated by rabbit anti-MPP11 antibody A303-721A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) a... View More
Product Details
A303-722A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 571 and 621 of human DnaJ (Hsp40) Homolog, Subfamily C, Member 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DnaJ (Hsp40) homolog, subfamily C, member 2; DnaJ homolog subfamily C member 2; M-phase phosphoprotein 11; Zuotin-related factor 1
Gene Aliases: DNAJC2; MPHOSPH11; MPP11; ZRF1; ZUO1
UniProt ID: (Human) Q99543
Entrez Gene ID: (Human) 27000
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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