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Invitrogen
MPP2 Polyclonal Antibody
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FIGURE: 1 / 1
MPP2 Antibody (PA5-95384) in WB
Western blot analysis of MPP2 using MPP2 Polyclonal Antibody (Product # PA5-95384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat brain tissue lysates. Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was in... View More
Product Details
PA5-95384
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human MPP2 recombinant protein (Position: R387-Y576).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human MPP2 shares 96.8% amino acid (aa) sequence identity with mouse MPP2.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The MAGUK (membrane-associated guanylate kinase homologs) family of proteins contain multiple protein-binding domains and are involved in cell junction organization, tumor suppression and signaling. The MAGUK family is divided into four subfamilies: DLG-like, ZO1-like, p55-like and LIN2-like. MPP2 (membrane protein, palmitoylated 2), also known as MAGUK p55 subfamily member 2, discs large homolog 2 or DLG2, is a 576 amino acid protein belonging to the MAGUK family that exists as three alternatively spliced isoforms. MPP2 contains one guanylate kinase-like domain, a PDZ (DHR) domain, two L27 domains and a single SH3 domain. The gene encoding MPP2 maps to the same segment of human chromosome 17 as MPP3, with whom MMP2 likely shares similar function and common structural organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Discs large homolog 2; discs large, homolog 2; MAGUK p55 subfamily member 2; membrane palmitoylated protein 2; membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2); p55 subfamily member of MAGUK family; Protein MPP2; unnamed protein product
Gene Aliases: D11Bwg0652e; DLG2; Dlgh2; MPP2; Pals4; rCG_34691
UniProt ID: (Human) Q14168, (Mouse) Q9WV34, (Rat) D3ZAA9
Entrez Gene ID: (Human) 4355, (Mouse) 50997, (Rat) 85275
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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