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MPR 300 Polyclonal Antibody
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FIGURE: 1 / 4
MPR 300 Antibody (OSI00001W-100UL) in IHC (P)
IHC-P on paraffin sections of human liver. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
Product Details
OSI00001W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from human MPR 300 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in rat and mouse.
Specificity of this antibody: MPRI.
Target Information
Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 300 kDa mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor; Cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI Man-6-P receptor; IGF-II receptor; IGF-II/CI-MPR; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6P/IGF2R; M6PR; mannose 6-phosphate/insulin-like growth factor-II receptor; mannose-6-phosphate receptor, cation independent; MPR 300; unnamed protein product
Gene Aliases: AI661837; CD222; CI-M6PR; CI-MPR; CIMPR; IGF2R; Igfr2; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI
UniProt ID: (Human) P11717, (Mouse) Q07113
Entrez Gene ID: (Human) 3482, (Mouse) 16004, (Rat) 25151
G-protein alpha-subunit binding
retinoic acid binding
insulin-like growth factor-activated receptor activity
protein binding
insulin-like growth factor binding
mannose binding
enzyme binding
insulin-like growth factor II binding
signaling receptor activity
identical protein binding
phosphoprotein binding
retromer complex binding
glycoprotein binding
G-protein coupled receptor activity
transporter activity
membrane traffic protein
liver development
receptor-mediated endocytosis
lysosomal transport
signal transduction
G-protein coupled receptor signaling pathway
spermatogenesis
post-embryonic development
organ regeneration
response to retinoic acid
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation by host of viral process
insulin-like growth factor receptor signaling pathway
response to tetrachloromethane
transport
protein transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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