|Tested species reactivity||Chicken, Hamster, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human Mre11 full length.|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunoprecipitation (IP)||3 ul|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473) Immunoprecipitation and Western Blot.
In WB, a band can be seen at ~81 kDa.
For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 ul for immunoprecipitation of 3X10^6 cells.
Co-IP application has been reported by Ching et al 2012 (PMID: 22190719).
Suggested positive control: antigen standard for MRE11A (transient overexpression lysate), Hela whole cell extract, Hela whole cell lysate.
The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA-Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity. HMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.