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FIGURE: 1 / 1
MRPL1 Antibody (PA5-109974) in WB
Western blot analysis of MRPL1 in extracts of various cell lines. Samples were incubated with MRPL1 Polyclonal antibody (Product # PA5-109974) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-109974
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 51-325 of human MRPL1 (NP_064621.3).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.57 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855385
Product Specific Information
Immunogen sequence: KKTKKGAKEK TPDEKKDEIE KIKAYPYMEG EPEDDVYLKR LYPRQIYEVE KAVHLLKKFQ ILDFTSPKQS VYLDLTLDMA LGKKKNVEPF TSVLSLPYPF ASEINKVAVF TENASEVKIA EENGAAFAGG TSLIQKIWDD EIVADFYVAV PEIMPELNRL RKKLNKKYPK LSRNSIGRDI PKMLELFKNG HEIKVDEERE NFLQTKIATL DMSSDQIAAN LQAVINEVCR HRPLNLGPFV VRAFLRSSTS EGLLLKIDPL LPKEVKNEES EKEDA
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39S ribosomal protein L1, mitochondrial; L1mt; Large ribosomal subunit protein uL1m
Gene Aliases: BM-022; BM022; L1MT; MRP-L1; MRPL1
UniProt ID: (Human) Q9BYD6
Entrez Gene ID: (Human) 65008, (Rat) 289491
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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