Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of MRPL12 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a MRPL12 polyclonal antibody (Product # PA5-30159) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 198 of Human MRPL12|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30159 targets MRPL12 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-30159 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 198 of Human MRPL12.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
39S ribosomal protein L12; 39S ribosomal protein L12, mitochondrial; 5c5-2; L12mt; mitochondrial; MRP-L12; MRP-L31/34; MRPL7; MRPL7/L12; RPML12
0610034O11Rik; 1500031N16Rik; 5c5-2; L12mt; MRP-L12; MRP-L31/34; MRPL12; MRPL7; MRPL7/L12; RPML12