|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 198 of Human MRPL12|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-30159 targets MRPL12 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-30159 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 198 of Human MRPL12.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 39S ribosomal protein L12; 39S ribosomal protein L12, mitochondrial; 5c5-2; L12mt; mitochondrial; Mitochondrial large ribosomal subunit protein bL12m; MRP-L12; MRP-L31/34; MRPL7; MRPL7/L12; RPML12
Gene Aliases: 0610034O11Rik; 1500031N16Rik; 5c5-2; L12mt; MRP-L12; MRP-L31/34; MRPL12; MRPL7; MRPL7/L12; RPML12
Molecular Function: RNA binding protein amino acid transporter calcium-binding protein calmodulin intracellular calcium-sensing protein mitochondrial carrier protein nucleic acid binding ribosomal protein transfer/carrier protein transporter
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