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MRPL18 Antibody (PA5-109976) in WB
Western blot analysis of MRPL18 in extracts of various cell lines. Samples were incubated with MRPL18 Polyclonal antibody (Product # PA5-109976) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-109976
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-180 of mouse Mrpl18 (NP_0805861)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.7 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855387
Product Specific Information
Immunogen sequence: EAVAPEFTNR NPRNLELLGV ARKERGWATV WPNREFWHRL RVVKTQHHVE AFVEHLNGQV VVSASTREWA IKKHLYSTRN VVACESIGRV LAQRCLEAGI NFMVYQPTPW EASSDSIKRL QNAMTESGVM LREPRRIYE
Target Information
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39S ribosomal protein L18, mitochondrial; L18mt; Large ribosomal subunit protein uL18m
Gene Aliases: 1010001C05Rik; C79879; HSPC071; L18mt; MRP-L18; MRPL18
UniProt ID: (Human) Q9H0U6, (Mouse) Q9CQL5
Entrez Gene ID: (Human) 29074, (Mouse) 67681
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