Proteintech

MRPL46 Polyclonal Antibody

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MRPL46 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MRPL46 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta using 16611-1-AP (MRPL46 antibody) at dilution of 1:50 (under 40x lens).

MRPL46 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 16611-1-AP (MRPL46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Product Details

16611-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPL46 Fusion Protein Ag9837 (1-279 aa encoded by BC017883) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAAPVRRTL LGVAGGWRRF ERLWAGSLSS RSLALAAAPS SNGSPWRLLG ALCLQRPPVV SKPLTPLQEE MASLLQQIEI ERSLYSDHEL RALDENQRLA KKKADLHDEE DEQDILLAQD LEDMWEQKFL QFKLGARITE ADEKNDRTSL NRKLDRNLVL LVREKFGDQD VWILPQAEWQ PGETLRGTAE RTLATLSENN MEAKFLGNAP CGHYTFKFPQ AMRTESNLGA KVFFFKALLL TGDFSQAGNK GHHVWVTKDE LGDYLKPKYL AQVRRFVSDL (1-279 aa encoded by BC017883)

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.