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MRPL49 Polyclonal Antibody

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MRPL49 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MRPL49 Antibody in Western Blot (WB) — BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

MRPL49 Antibody in Immunoprecipitation (IP) — IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug.

Product Details

15542-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPL49 Fusion Protein Ag7898 (1-166 aa encoded by BC004378) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAATMFRAT LRGWRTGVQR GCGLRLLSQT QGPPDYPRFV ESVDEYQFVE RLLPATRIPD PPKHEHYPTP SGWQPPRDPP PNLPYFVRRS RMHNIPVYKD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRI KGYFDQELKA WLLEKGF (1-166 aa encoded by BC004378)

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for the HRD1 protein use in their respective 3' UTRs some of the same genomic sequence. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure.

Scientific reports

Gerhold JM,Cansiz-Arda Ş,Lõhmus M,Engberg O,Reyes A,van Rennes H,Sanz A,Holt IJ,Cooper HM,Spelbrink JN

Mon Oct 19 00:00:00 EDT 2015

Largen: a molecular regulator of mammalian cell size control.

Molecular cell

Yamamoto K,Gandin V,Sasaki M,McCracken S,Li W,Silvester JL,Elia AJ,Wang F,Wakutani Y,Alexandrova R,Oo YD,Mullen PJ,Inoue S,Itsumi M,Lapin V,Haight J,Wakeham A,Shahinian A,Ikura M,Topisirovic I,Sonenberg N,Mak TW

Thu Mar 20 00:00:00 EDT 2014

Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.

Nucleic acids research

Rajala N,Gerhold JM,Martinsson P,Klymov A,Spelbrink JN

Wed Jan 01 00:00:00 EST 2014

C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.

Nucleic acids research

Wanschers BF,Szklarczyk R,Pajak A,van den Brand MA,Gloerich J,Rodenburg RJ,Lightowlers RN,Nijtmans LG,Huynen MA

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 39S ribosomal protein L49, mitochondrial; L49mt; MGC10656; Mitochondrial large ribosomal subunit protein mL49; Neighbor of FAU; next to FAU; NOF; Protein NOF1

Gene Aliases: C11orf4; L49mt; MRP-L49; MRPL49; NOF; NOF1; OK/SW-cl.67

UniProt ID: (Human) Q13405

Entrez Gene ID: (Human) 740

Molecular Function: ribosomal protein translational protein

Function(s)

mitochondrial translational elongation mitochondrial translational termination

Process(es)

structural constituent of ribosome protein binding

MRPL49 Polyclonal Antibody

Product Details

15542-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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