Invitrogen

MRPL57 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (2) MRPL57 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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MRPL57 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MRPL57 in human adrenal gland and pancreas tissues using MRPL57 Polyclonal Antibody (Product # PA5-58926). Corresponding MRPL57 RNA-seq data are presented for the same tissues.

MRPL57 Antibody in Western Blot (WB) — Western blot analysis of MRPL57 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MRPL57 Polyclonal Antibody (Product # PA5-58926).

MRPL57 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MRPL57 demonstrates antibody specificity. Immunohistochemical analysis of MRPL57 using anti-MRPL57 Polyclonal Antibody (Product # PA5-58926), shows significant staining of MRPL57 in human adrenal gland and shows minimal or weak staining in human human pancreas tissues. The relative expression levels of MRPL57 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-58926

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human MRPL57 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644169

Product Specific Information

Immunogen sequence: RFVSLRAKQN MIRRLEIEAE NHYWLSMPYM TREQERGHAA VRRREAFEAI KA

Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 81%.

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein which belongs to an undetermined ribosomal subunit and which seems to be specific to animal mitoribosomes. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 3p, 5q, 8q, 14q, and Y.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hMRP63; Mitochondrial large ribosomal subunit protein mL63; Mitochondrial ribosomal protein 63; mitochondrial ribosomal protein bMRP63; Mitochondrial ribosomal protein L57; Ribosomal protein 63, mitochondrial

Gene Aliases: bMRP63; MRP63; MRPL57

UniProt ID: (Human) Q9BQC6

Entrez Gene ID: (Human) 78988

Molecular Function: ribosomal protein translational protein