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Invitrogen
MSH3 Polyclonal Antibody
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FIGURE: 1 / 3
MSH3 Antibody (PA5-114764) in ICC/IF
Immunocytochemistry analysis of MSH3 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114764). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-114764
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MSH3(Accession P20585), corresponding to amino acid residues G110-F160.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MSH3.
Target Information
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Divergent upstream protein; DNA mismatch repair protein Msh3; DUP; epididymis secretory sperm binding protein; hMSH3; Mismatch repair protein 1; MRP1; Protein repair-1; Protein repair-3; REP-1; REP-3; unnamed protein product
Gene Aliases: D13Em1; DUC1; DUG; DUP; FAP4; MRP1; MSH3; Rep-3; Rep3
UniProt ID: (Human) P20585, (Mouse) P13705
Entrez Gene ID: (Human) 4437, (Rat) 499505, (Mouse) 17686
nucleotide binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
enzyme binding
mismatched DNA binding
guanine/thymine mispair binding
dinucleotide insertion or deletion binding
single guanine insertion binding
dinucleotide repeat insertion binding
oxidized purine DNA binding
protein homodimerization activity
ATP-dependent DNA damage sensor activity
Y-form DNA binding
heteroduplex DNA loop binding
double-strand/single-strand DNA junction binding
molecular_function
damaged DNA binding
DNA-dependent ATPase activity
centromeric DNA binding
DNA metabolism protein
DNA repair
mismatch repair
mitotic recombination
cellular response to DNA damage stimulus
somatic recombination of immunoglobulin gene segments
maintenance of DNA repeat elements
negative regulation of DNA recombination
meiotic mismatch repair
reciprocal meiotic recombination
biological_process
somatic hypermutation of immunoglobulin genes
positive regulation of helicase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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