Western blot analysis of MSH3 using A) 30 µg A549 whole cell lysate (B) 30 µg H1299 whole cell lysate and C) 30 µg HCT116 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MSH3 polyclonal antibody (Product # PA5-27864) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 59 and 349 of Human MSH3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27864 targets MSH3 in WB applications and shows reactivity with Human samples.
The PA5-27864 immunogen is recombinant fragment corresponding to a region within amino acids 59 and 349 of Human MSH3.
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.