Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is rectal carcinoma.
MSH3 belongs to the MutS DNA mismatch repair family although its precise function in mammals is still unknown. Yeast MSH3 is thought to act in a complex with MSH2 to recognize DNA insertion/deletion mispairs.
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Protein Aliases: Divergent upstream protein; DNA mismatch repair protein Msh3; DUP; hMSH3; Mismatch repair protein 1; MRP1
Gene Aliases: DUC1; DUG; DUP; MRP1; MSH3
UniProt ID: (Human) P20585
Entrez Gene ID: (Human) 4437