Invitrogen

MSH6 Polyclonal Antibody

Product Image Gallery

MSH6 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MSH6 in U-2 OS cells using a MSH6 polyclonal antibody (Product # PA5-83010). The analysis shows localization to nucleoplasm and vesicles.

MSH6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of MSH6 in human lymph node using a MSH6 polyclonal antibody (Product # PA5-83010). The analysis shows strong nuclear positivity in reaction center cells.

MSH6 Antibody in Western Blot (WB) — Western blot analysis of MSH6 by a MSH6 polyclonal antibody (Product # PA5-83010). Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Mouse, Rat, Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MSH6

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Immunogen sequence: MVENECEDPS QETITFLYKF IKGACPKSYG FNAARLANLP EEVIQKGHRK AREFEKMNQS LRLFREVCLA SERSTV

Target Information

The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; hMSH6; mutS homolog 6; MutS protein homolog 6; MutS-alpha 160 kDa subunit; p160; rCG61559-like; sperm-associated protein

Gene Aliases: AU044881; AW550279; GTBP; GTMBP; HNPCC5; HSAP; MSH6; p160

UniProt ID: (Human) P52701, (Mouse) P54276

Entrez Gene ID: (Rat) 100360342, (Human) 2956, (Mouse) 17688

Molecular Function: DNA binding protein nucleic acid binding