|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 331-380 of human MSK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 90 kDa.
Purity is >95% by SDS-PAGE.
MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 90 kDa ribosomal protein S6 kinase 5; mitogen and stress-activated protein kinase 1; MSK1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLSK; RPS6KA5; RSK-like protein kinase; RSKL; S6K-alpha-5
Gene Aliases: 3110005L17Rik; 6330404E13Rik; AI854034; MSK1; MSPK1; RLPK; RPS6KA5
Molecular Function: annexin calcium-binding protein calmodulin cytoskeletal protein intracellular calcium-sensing protein kinase microtubule family cytoskeletal protein non-motor microtubule binding protein non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase
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