Invitrogen

MSX2 Polyclonal Antibody

View all (8) MSX2 antibodies

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MSX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of MSX2 in paraffin embedded human ovarian cancer using a MSX2 polyclonal antibody (Product # PA5-97984) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

MSX2 Antibody in Western Blot (WB) — Western Blot analysis of MSX2 using a MSX2 Polyclonal antibody (Product # PA5-97984) at a concentration of 2 µg/mL. Positive WB detected in: Jurkat whole cell lysate, Rat kidney tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 29 kDa.

Product Details

PA5-97984

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Homeobox protein MSX-2 protein (50-120AA) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	4.71 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% ProClin 300
Storage conditions	-20°C or -80°C if preferred
RRID	AB_2812598

Target Information

Msx2 is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. This protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in the Msx2 gene are associated with parietal foramina 1 and craniosynostosis type 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein Hox-8; homeobox protein Hox-8-1; Homeobox protein MSX-2; hox-8.1; msh homeo box 2; msh homeo box homolog 2; msh homeobox homolog 2

Gene Aliases: CRS2; FPP; HOX8; MSH; MSX2; PFM; PFM1

UniProt ID: (Human) P35548

Entrez Gene ID: (Human) 4488, (Rat) 25483

Molecular Function: homeodomain transcription factor

negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation chondrocyte development osteoblast development outflow tract septum morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation endochondral bone growth transcription from RNA polymerase II promoter negative regulation of cell proliferation anterior/posterior pattern specification signal transduction involved in regulation of gene expression positive regulation of BMP signaling pathway negative regulation of CREB transcription factor activity embryonic forelimb morphogenesis embryonic hindlimb morphogenesis wound healing, spreading of epidermal cells embryonic nail plate morphogenesis negative regulation of apoptotic process negative regulation of fat cell differentiation negative regulation of keratinocyte differentiation positive regulation of osteoblast differentiation negative regulation of transcription, DNA-templated stem cell differentiation positive regulation of catagen bone trabecula formation cranial suture morphogenesis frontal suture morphogenesis branching involved in mammary gland duct morphogenesis BMP signaling pathway involved in heart development enamel mineralization cellular response to estradiol stimulus activation of meiosis negative regulation of transcription regulatory region DNA binding positive regulation of mesenchymal cell apoptotic process ossification outflow tract morphogenesis embryonic limb morphogenesis BMP signaling pathway wound healing odontogenesis embryonic digit morphogenesis regulation of apoptotic process positive regulation of apoptotic process cartilage development bone morphogenesis mammary gland epithelium development cellular response to growth factor stimulus

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription cofactor activity protein binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding transcription factor activity, transcription factor binding DNA binding