|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 650-700 of human MTA2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with mouse, rat, canine, bovine, and xenopus samples based on sequence homology.
Suggested positive control: Jurkat cell lysate.
MTA2, a homologue of human MTA1 and MTA3, is a member of highly conserved MTA gene family. It is a component of NuRD, an ATP-dependent nucleosome remodeling and histone deacetylase complex. MTA2 is a nuclear protein that interacts with HDAC1 and HDAC2 and has a functional role in chromatin remodeling and deacetylase activity. MTA2 specifically interacts with p53 and represses p53-dependant transcriptional activation, thereby regulating p53-mediated cell growth arrest and apoptosis. It also inhibits the transcriptional activity of Estrogen receptor-alpha. Northern Blot analysis detected a ubiquitous expression of MTA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The chromatin remodeling factor CHD5 is a transcriptional repressor of WEE1.
PA1-41581 was used in western blot to determine how CHD5 suppresses tumors
|Quan J,Adelmant G,Marto JA,Look AT,Yusufzai T||PloS one (9:null)||2014|