Proteintech

MTAP Polyclonal Antibody

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MTAP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed NIH/3T3 cells using 11475-1-AP (MTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MTAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 11475-1-AP (MTAP antibody) at dilution of 1:50 (under 10x lens).

MTAP Antibody in Western Blot (WB) — Various lysates from MTAP-expressing cells (Raji, NIH3T3, HeLa, and HEK293) and MTAP deficient cells (MCF7 and A549) were subjected to SDS PAGE followed by western blot with 11475-1-AP (MTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Product Details

11475-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MTAP Fusion Protein Ag2051 (1-154 aa encoded by BC018625) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.37 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MASGTTTTA VKIGIIGGTG LDDPEILEGR TEKYVDTPFG KPSDALILGK IKNVDCILLA RHGRQHTIMP SKVNYQANIW ALKEEGCTHV IVTTACGSLR EEIQPGDIVI IDQFIDSHVR RACFPFTFHH DCFQRPPQKP SRSHCVSCAT CRTMS (1-154 aa encoded by BC018625)

Target Information

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SHARPIN-mediated regulation of protein arginine methyltransferase 5 controls melanoma growth.

The Journal of clinical investigation

Tamiya H,Kim H,Klymenko O,Kim H,Feng Y,Zhang T,Han JY,Murao A,Snipas SJ,Jilaveanu L,Brown K,Kluger H,Zhang H,Iwai K,Ronai ZA

Tue Jan 02 00:00:00 EST 2018

Loss of MTAP expression is a negative prognostic marker in Ewing sarcoma family of tumors.

Biomarkers in medicine

Abrahao-Machado LF,Antunes B,Filippi RZ,Volc S,Boldrini E,Menezes WP,Reis RM,de Camargo OP

Mon Jan 01 00:00:00 EST 2018

Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model.

Oncotarget

Affronti HC,Long MD,Rosario SR,Gillard BM,Karasik E,Boerlin CS,Pellerite AJ,Foster BA,Attwood K,Pili R,Wilton JH,Campbell MJ,Smiraglia DJ

Tue Nov 28 00:00:00 EST 2017

Suppressive effects of tumor cell-derived 5'-deoxy-5'-methylthioadenosine on human T cells.

Oncoimmunology

Henrich FC,Singer K,Poller K,Bernhardt L,Strobl CD,Limm K,Ritter AP,Gottfried E,Völkl S,Jacobs B,Peter K,Mougiakakos D,Dettmer K,Oefner PJ,Bosserhoff AK,Kreutz MP,Aigner M,Mackensen A

Mon Aug 01 00:00:00 EDT 2016

Expression of Methylthioadenosine Phosphorylase (MTAP) in Pilocytic Astrocytomas.

Pathobiology : journal of immunopathology, molecular and cellular biology

Becker AP,Scapulatempo-Neto C,Menezes WP,Clara C,Machado HR,Oliveira RS,Neder L,Reis RM

Mon May 09 00:00:00 EDT 2016

Characterization and Prognostic Significance of Methylthioadenosine Phosphorylase Deficiency in Nasopharyngeal Carcinoma.

Medicine

He HL,Lee YE,Shiue YL,Lee SW,Chen TJ,Li CF

Tue Dec 01 00:00:00 EST 2015

Integrated omic analysis of oropharyngeal carcinomas reveals human papillomavirus (HPV)-dependent regulation of the activator protein 1 (AP-1) pathway.

Molecular & cellular proteomics : MCP

Sepiashvili L,Waggott D,Hui A,Shi W,Su S,Ignatchenko A,Ignatchenko V,Laureano M,Huang SH,Xu W,Weinreb I,Waldron J,O'Sullivan B,Irish JC,Boutros PC,Liu FF,Kislinger T

Mon Dec 01 00:00:00 EST 2014

Downregulated MTAP expression in myxofibrosarcoma: A characterization of inactivating mechanisms, tumor suppressive function, and therapeutic relevance.

Oncotarget

Li CF,Fang FM,Kung HJ,Chen LT,Wang JW,Tsai JW,Yu SC,Wang YH,Li SH,Huang HY

Sun Nov 30 00:00:00 EST 2014

Downregulated MTAP expression in myxofibrosarcoma: A characterization of inactivating mechanisms, tumor suppressive function, and therapeutic relevance.

Oncotarget

Li CF,Fang FM,Kung HJ,Chen LT,Wang JW,Tsai JW,Yu SC,Wang YH,Li SH,Huang HY

Sun Nov 30 00:00:00 EST 2014

Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region.

PloS one

Chidlow G,Wood JP,Sharma S,Dimasi DP,Burdon KP,Casson RJ,Craig JE

Sun Jun 15 00:00:00 EDT 2014

Ocular expression and distribution of products of the POAG-associated chromosome 9p21 gene region.

PloS one

Chidlow G,Wood JP,Sharma S,Dimasi DP,Burdon KP,Casson RJ,Craig JE

Sun Jun 15 00:00:00 EDT 2014

Significantly decreased methylthioadenosine phosphorylase expression in malignant melanoma.

The American Journal of dermatopathology

Gambichler T,Scola N,Bechara FG

Mon Oct 01 00:00:00 EDT 2012

The diagnostic value of immunohistochemically detected methylthioadenosine phosphorylase deficiency in malignant pleural mesotheliomas.

Histopathology

Zimling ZG,Jørgensen A,Santoni-Rugiu E

Tue May 01 00:00:00 EDT 2012

Downregulation of methylthioadenosin phosphorylase by homozygous deletion in gastric carcinoma.

Genes, chromosomes & cancer

Kim J,Kim MA,Min SY,Jee CD,Lee HE,Kim WH

Wed Jun 01 00:00:00 EDT 2011

Downregulation of methylthioadenosin phosphorylase by homozygous deletion in gastric carcinoma.

Genes, chromosomes & cancer

Kim J,Kim MA,Min SY,Jee CD,Lee HE,Kim WH

Wed Jun 01 00:00:00 EDT 2011

Methylthioadenosine phosphorylase and activated insulin-like growth factor-1 receptor/insulin receptor: potential therapeutic targets in chordoma.

The Journal of pathology

Sommer J,Itani DM,Homlar KC,Keedy VL,Halpern JL,Holt GE,Schwartz HS,Coffin CM,Kelley MJ,Cates JM

Thu Apr 01 00:00:00 EDT 2010

Homozygous deletion of MTAP gene as a poor prognosticator in gastrointestinal stromal tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research

Huang HY,Li SH,Yu SC,Chou FF,Tzeng CC,Hu TH,Uen YH,Tian YF,Wang YH,Fang FM,Huang WW,Wei YC,Wu JM,Li CF

Sun Nov 15 00:00:00 EST 2009

Bioinformatics

Protein Aliases: 5'-methylthioadenosine phosphorylase; epididymis luminal protein 249; MeSAdo phosphorylase; MTA phosphorylase; MTAP; MTAPase; S-methyl-5'-thioadenosine phosphorylase

Gene Aliases: 1300019I21Rik; BDMF; c86fus; Cc1-6; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP; MTAP

UniProt ID: (Human) Q13126, (Mouse) Q9CQ65

Entrez Gene ID: (Human) 4507, (Mouse) 66902, (Rat) 298227

Molecular Function: nucleotide kinase