Invitrogen

MTCH2 Polyclonal Antibody

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MTCH2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using a MTCH2 polyclonal antibody (Product # PA5-25406), followed by HRP-conjugated secondary antibody and DAB staining.

MTCH2 Antibody in Western Blot (WB) — Western blot analysis using a MTCH2 polyclonal antibody (Product # PA5-25406) in mouse NIH-3T3 cell lysates (35 µg per lane).

Product Details

PA5-25406

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human MTCH2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2542906

Target Information

MTCH2 is a member of the mitochondrial carrier protein family that catalyze the exchange of substrates across the inner mitochondrial membrane and is targeted by Bid, a pro-apoptotic Bcl-2 family member, in response to apoptotic signals, suggesting that MTCH2 may play a key role in the mitochondrial apoptosis pathway. Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential.

The Journal of biological chemistry

Manjunath LE,Singh A,Sahoo S,Mishra A,Padmarajan J,Basavaraju CG,Eswarappa SM

PA5-25406 was used in Western Blotting to demonstrate translational read-through across two evolutionarily conserved, in-frame stop codons of MTCH2 using luminescence- and fluorescence-based assays, and by analyzing ribosome-profiling and mass spectrometry (MS) data.