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Invitrogen
MTCO2 Recombinant Rabbit Monoclonal Antibody (24GB3270)
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FIGURE: 1 / 3
MTCO2 Antibody (MA5-57085) in ICC/IF
Immunocytochemical staining of MTCO2 in HepG2 cells using MTCO2 Recombinant Monoclonal Antibody (Product # MA5-57085) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; MTCO2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-57085
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB3270
Immunogen
A synthesized peptide derived from human MTCO2 (aa 180-227)
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Co2; cyclooxygenase; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2
Gene Aliases: COII; COX2; COXII; MT-CO2; MTCO2
UniProt ID: (Human) P00403
Entrez Gene ID: (Human) 4513
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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