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Invitrogen
MTCO2 Monoclonal Antibody (4B12A5)
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FIGURE: 1 / 8
MTCO2 Antibody (459150) in WB
The Journal of cell biology 2022 -
Figure S4. mtDNA damage results in mitochondrial dysfunction. (A) Representative images of cells expressing Cox4::GFP stained with TMRE after treatment with FCCP (2 muM) for 20 min or Oligomycin (0.5 mug/ml) for 4 h (left panel). Total TMRE intensity normalized to cell area is plotted; n > 650 pooled from three biological replicates for FCCP and Oligomycin and two biological replicates for empty vector (rig... View More
Product Details
459150
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Published species
Yeast
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4B12A5
Immunogen
Full length purified native protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
IgG fraction
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: Mitochondria from yeast membrane extract
Target Information
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Co2; cyclooxygenase; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2
Gene Aliases: ACI60_gp02; COX2; OXI1; OXII; Q0250
UniProt ID: (Baker's yeast) P00410
Entrez Gene ID: (Baker's yeast) 854622, (Baker's yeast) 24573139
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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