Proteintech

MTF2 Polyclonal Antibody

MTF2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

MTF2 Antibody (16208-1-AP) in ICC/IF

IF result of mouse ES cell From Kevin Corbit.

MTF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MTF2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16208-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

ChIP assay (ChIP)

View 4 publications 4 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MTF2 Fusion Protein Ag9182 (187-536 aa encoded by BC010013)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ADLEWDAGH KTNVQQCYCY CGGPGDWYLK MLQCCKCKQW FHEACVQCLQ KPMLFGDRFY TFICSVCSSG PEYLKRLPLQ WVDIAHLCLY NLSVIHKKKY FDSELELMTY INENWDRLHP GELADTPKSE RYEHVLEALN DYKTMEVSNG IEKKGKKKSV GRPPGPYTRK MIQKTAEPLL DKESISENPT LDLPCSIGRT EGTAHSSNTS DVDFTGASSA KETTSSSISR HYGLSDSRKR TRTGRSWPAA IPHLRRRRGR LPRRALQTQN SEIVKDDEGK EDYQFDELNT EILNNLADQE LQLNHLKNSI TSYFGAAGRI ACGEKYRVLA RRVTLDGKVQ YLVEWEGATA S (187-536 aa encoded by BC010013)

Target Information

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes.

Nature cell biology

Sparbier CE,Gillespie A,Gomez J,Kumari N,Motazedian A,Chan KL,Bell CC,Gilan O,Chan YC,Popp S,Gough DJ,Eckersley-Maslin MA,Dawson SJ,Lehner PJ,Sutherland KD,Ernst P,McGeehan GM,Lam EYN,Burr ML,Dawson MA

Wed Feb 01 00:00:00 EST 2023

Combined inhibition of EZH2 and the autotaxin-LPA-LPA2 axis exerts synergistic antitumor effects on colon cancer cells.

Cancer letters

Long Y,Wang Y,Qu M,Zhang D,Zhang X,Zhang J

Mon Jul 10 00:00:00 EDT 2023

Identification of the Transcription Co-Factor-Related Gene Signature and Risk Score Model for Osteosarcoma.

Frontiers in genetics

Jin Z,Wu J,Lin J,Wang J,Shen Y

Sat Jul 16 00:00:00 EDT 2022

The transcriptional elongation factor CTR9 demarcates PRC2-mediated H3K27me3 domains by altering PRC2 subtype equilibrium.

Nucleic acids research

Chan NT,Huang J,Ma G,Zeng H,Donahue K,Wang Y,Li L,Xu W

Mon Feb 28 00:00:00 EST 2022

The transcriptional elongation factor CTR9 demarcates PRC2-mediated H3K27me3 domains by altering PRC2 subtype equilibrium.

Nucleic acids research

Chan NT,Huang J,Ma G,Zeng H,Donahue K,Wang Y,Li L,Xu W

Mon Feb 28 00:00:00 EST 2022

JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation.

Cell reports

Tavares M,Khandelwal G,Muter J,Viiri K,Beltran M,Brosens JJ,Jenner RG

Tue May 31 00:00:00 EDT 2022

JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation.

Cell reports

Tavares M,Khandelwal G,Muter J,Viiri K,Beltran M,Brosens JJ,Jenner RG

Tue May 31 00:00:00 EDT 2022

PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing.

Molecular cell

Fong KW,Zhao JC,Lu X,Kim J,Piunti A,Shilatifard A,Yu J

Thu Dec 15 00:00:00 EST 2022

Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

Frontiers in cell and developmental biology

García-Montolio M,Ballaré C,Blanco E,Gutiérrez A,Aranda S,Gómez A,Kok CH,Yeung DT,Hughes TP,Vizán P,Di Croce L

Tue May 18 00:00:00 EDT 2021

Distinct PRC2 subunits regulate maintenance and establishment of Polycomb repression during differentiation.

Molecular cell

Petracovici A,Bonasio R

Thu Jun 17 00:00:00 EDT 2021

Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.

Molecular cell

Youmans DT,Gooding AR,Dowell RD,Cech TR

Thu Feb 04 00:00:00 EST 2021

Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.

Molecular cell

Youmans DT,Gooding AR,Dowell RD,Cech TR

Thu Feb 04 00:00:00 EST 2021

BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation.

Molecular cell

Conway E,Rossi F,Fernandez-Perez D,Ponzo E,Ferrari KJ,Zanotti M,Manganaro D,Rodighiero S,Tamburri S,Pasini D

Thu Sep 02 00:00:00 EDT 2021

A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin.

Molecular cell

Chen S,Jiao L,Liu X,Yang X,Liu X

Thu Mar 19 00:00:00 EDT 2020

RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells.

Nature genetics

Long Y,Hwang T,Gooding AR,Goodrich KJ,Rinn JL,Cech TR

Tue Sep 01 00:00:00 EDT 2020

Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression.

Molecular cell

Tamburri S,Lavarone E,Fernández-Pérez D,Conway E,Zanotti M,Manganaro D,Pasini D

Thu Feb 20 00:00:00 EST 2020

Cell Signaling Coordinates Global PRC2 Recruitment and Developmental Gene Expression in Murine Embryonic Stem Cells.

iScience

Aljazi MB,Gao Y,Wu Y,Mias GI,He J

Fri Nov 20 00:00:00 EST 2020

PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells.

eLife

Jain P,Ballare C,Blanco E,Vizan P,Di Croce L

Tue Mar 10 00:00:00 EDT 2020

PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells.

eLife

Jain P,Ballare C,Blanco E,Vizan P,Di Croce L

Tue Mar 10 00:00:00 EDT 2020

Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells.

Journal of neuro-oncology

Wang F,Gao Y,Lv Y,Wu Y,Guo Y,Du F,Wang S,Yu J,Cao X,Li PA

Mon Jun 01 00:00:00 EDT 2020

PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation.

Molecular cell

Healy E,Mucha M,Glancy E,Fitzpatrick DJ,Conway E,Neikes HK,Monger C,Van Mierlo G,Baltissen MP,Koseki Y,Vermeulen M,Koseki H,Bracken AP

Thu Nov 07 00:00:00 EST 2019

Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity.

Nature communications

Lavarone E,Barbieri CM,Pasini D

Thu Apr 11 00:00:00 EDT 2019

Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity.

Nature communications

Lavarone E,Barbieri CM,Pasini D

Thu Apr 11 00:00:00 EDT 2019

G-tract RNA removes Polycomb repressive complex 2 from genes.

Nature structural & molecular biology

Beltran M,Tavares M,Justin N,Khandelwal G,Ambrose J,Foster BM,Worlock KB,Tvardovskiy A,Kunzelmann S,Herrero J,Bartke T,Gamblin SJ,Wilson JR,Jenner RG

Tue Oct 01 00:00:00 EDT 2019

MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.

Nature genetics

Perino M,van Mierlo G,Karemaker ID,van Genesen S,Vermeulen M,Marks H,van Heeringen SJ,Veenstra GJC

Sun Jul 01 00:00:00 EDT 2018

MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.

Nature genetics

Perino M,van Mierlo G,Karemaker ID,van Genesen S,Vermeulen M,Marks H,van Heeringen SJ,Veenstra GJC

Sun Jul 01 00:00:00 EDT 2018

Capturing the Onset of PRC2-Mediated Repressive Domain Formation.

Molecular cell

Oksuz O,Narendra V,Lee CH,Descostes N,LeRoy G,Raviram R,Blumenberg L,Karch K,Rocha PP,Garcia BA,Skok JA,Reinberg D

Thu Jun 21 00:00:00 EDT 2018

A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.

Molecular cell

Conway E,Jerman E,Healy E,Ito S,Holoch D,Oliviero G,Deevy O,Glancy E,Fitzpatrick DJ,Mucha M,Watson A,Rice AM,Chammas P,Huang C,Pratt-Kelly I,Koseki Y,Nakayama M,Ishikura T,Streubel G,Wynne K,Hokamp K,McLysaght A,Ciferri C,Di Croce L,Cagney G,Margueron R,Koseki H,Bracken AP

Thu May 03 00:00:00 EDT 2018

Polycomb-like proteins link the PRC2 complex to CpG islands.

Nature

Li H,Liefke R,Jiang J,Kurland JV,Tian W,Deng P,Zhang W,He Q,Patel DJ,Bulyk ML,Shi Y,Wang Z

Thu Sep 14 00:00:00 EDT 2017

Polycomb-like proteins link the PRC2 complex to CpG islands.

Nature

Li H,Liefke R,Jiang J,Kurland JV,Tian W,Deng P,Zhang W,He Q,Patel DJ,Bulyk ML,Shi Y,Wang Z

Thu Sep 14 00:00:00 EDT 2017

Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.

PLoS genetics

Hunkapiller J,Shen Y,Diaz A,Cagney G,McCleary D,Ramalho-Santos M,Krogan N,Ren B,Song JS,Reiter JF

Tue Sep 04 00:00:00 EDT 2012

Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.

PLoS genetics

Hunkapiller J,Shen Y,Diaz A,Cagney G,McCleary D,Ramalho-Santos M,Krogan N,Ren B,Song JS,Reiter JF

Tue Sep 04 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: hPCl2; Metal regulatory transcription factor 2; metal response element DNA-binding protein M96; metal response element-binding transcription factor 2; Metal-response element DNA-binding protein M96; Metal-response element-binding transcription factor 2; mPCl2; polycomb-like 2; Polycomb-like protein 2; putative DNA binding protein; RP5-976O13.1; tudor domain containing 19A; Zinc-regulated factor 1; ZiRF1

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Gene Aliases: 9230112N11Rik; AA537621; C76717; dJ976O13.2; M96; MTF2; PCL2; RGD1304727; TDRD19A

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UniProt ID: (Human) Q9Y483, (Mouse) Q02395

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Entrez Gene ID: (Human) 22823, (Rat) 360905, (Mouse) 17765

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Function(s)

DNA binding zinc ion binding methylated histone binding molecular_function protein binding metal ion binding chromatin/chromatin-binding, or -regulatory protein

Process(es)

negative regulation of transcription from RNA polymerase II promoter segment specification covalent chromatin modification stem cell population maintenance negative regulation of gene expression, epigenetic positive regulation of transcription from RNA polymerase II promoter stem cell differentiation negative regulation of histone H3-K27 methylation positive regulation of histone H3-K27 methylation biological_process chromatin modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTF2 Polyclonal Antibody

MTF2 Antibody (16208-1-AP) in ICC/IF

Product Details

16208-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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