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Invitrogen
MTF2 Polyclonal Antibody
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FIGURE: 1 / 1
MTF2 Antibody (PA5-96571) in WB
Western blot analysis of MTF2 in extracts of various cell lines. Samples were incubated with MTF2 Polyclonal antibody (Product # PA5-96571) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-96571
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MTF2 (NP_0011578641)
Conjugate
Form
Liquid
Concentration
0.24 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MRDSTGAGNS LVHKRSPLRR NQKTPTSLTK LSLQDGHKAK KPACKFEEGQ DVLARWSDGL FYLGTIKKIN ILKQSCFIIF EDSSKSWVLW KDIQTGATGS GEMVCTICQE EYSEAPNEMV ICDKCGQGYH QLCHTPHIDS SVIDSDEKWL CRQCVFATTT KRGGALKKGP NAKALQVMKQ TLPYSVADLE; Positive Samples: HL-60, HepG2, Mouse brain, Rat brain; Cellular Location: Nucleus
Target Information
Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPCl2; Metal regulatory transcription factor 2; metal response element DNA-binding protein M96; metal response element-binding transcription factor 2; Metal-response element DNA-binding protein M96; Metal-response element-binding transcription factor 2; mPCl2; polycomb-like 2; Polycomb-like protein 2; putative DNA binding protein; RP5-976O13.1; tudor domain containing 19A; Zinc-regulated factor 1; ZiRF1
Gene Aliases: 9230112N11Rik; AA537621; C76717; dJ976O13.2; M96; MTF2; PCL2; RGD1304727; TDRD19A
UniProt ID: (Human) Q9Y483, (Mouse) Q02395
Entrez Gene ID: (Human) 22823, (Rat) 360905, (Mouse) 17765
negative regulation of transcription from RNA polymerase II promoter
segment specification
covalent chromatin modification
stem cell population maintenance
negative regulation of gene expression, epigenetic
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
negative regulation of histone H3-K27 methylation
positive regulation of histone H3-K27 methylation
biological_process
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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