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Invitrogen
MTH1 Polyclonal Antibody
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FIGURE: 1 / 4
MTH1 Antibody (PA5-89163) in WB
Western blot analysis of MTH1 in extracts of various cell lines. Samples were incubated with MTH1 Polyclonal antibody (Product # PA5-89163) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-89163
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MTH1 (NP_9451921)
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Amount
94 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSGISPQQMG EPEGSWSGKN PGTMGASRLY TLVLVLQPQR VLLGMKKRGF GAGRWNGFGG KVQEGETIED GARRELQEES GLTVDALHKV GQIVFEFVGE PELMDVHVFC TDSIQGTPVE SDEMRPCWFQ LDQIPFKDMW PDDSYWFPLL LQKKKFHGYF KFQGQDTILD YTLREVDTV; Positive Samples: SW480, K562, 22RV1, THP-1, HepG2, SKOV3, 293T; Cellular Location: Cytoplasm, Mitochondrion matrix, Nucleus
Target Information
Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine and G:C to T:A transversion mutation occurs. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised from the DNA and excreted in the urine. Along with 8-oxoG being present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal cellular metabolism and following oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated in DNA during polymerization and can result in a mispairing unless repaired. MTH converts 8-oxo-dGTP in the nucleotide pool to the monophosphate and prevents the misincorporation of 8-oxo-dGTP into DNA (Figure courtesy of Dr. Mark Kelley). MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during gene transcription leading to missense or nonsense protein production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-hydroxy-dATP diphosphatase; 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; Methylated purine nucleoside triphosphate hydrolase; mutT human homolog 1; Nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1; nudix (nucleoside diphosphate linked moiety X)-type motif 1; Nudix motif 1; NUDT1; Oxidized purine nucleoside triphosphate hydrolase
Gene Aliases: MTH1; NUDT1
UniProt ID: (Human) P36639
Entrez Gene ID: (Human) 4521
RNA binding
protein binding
8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity
dATP pyrophosphohydrolase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
snoRNA binding
8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity
metal ion binding
ATP diphosphatase activity
2-hydroxy-ATP hydrolase activity
2-hydroxy-dATP hydrolase activity
N6-methyl-(d)ATP hydrolase activity
O6-methyl-dGTP hydrolase activity
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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