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Bethyl Laboratories
MTHFD1 Polyclonal Antibody
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FIGURE: 1 / 2
MTHFD1 Antibody (A305-286A) in WB
Detection of human and mouse MTHFD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTHFD1 antibody (Product # A305-286A) (lot A305-286A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A305-286A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 150 to 200 of human C-1-tetrahydrofolate synthase, cytoplasmic.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat and Orangutan
Target Information
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase; C-1-tetrahydrofolate synthase, cytoplasmic; C1-tetrahydrofolate synthase; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; Epididymis secretory sperm binding protein; MDMCSF (EC 1.5.1.5; EC 3.5.4.9; EC 6.3.4.3); methenyltetrahydrofolate; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase; unnamed protein product
Gene Aliases: CIMAH; Dcs; E430024A07Rik; MTHFC; MTHFD; MTHFD1
UniProt ID: (Human) P11586, (Mouse) Q922D8
Entrez Gene ID: (Human) 4522, (Mouse) 108156
nucleotide binding
catalytic activity
formate-tetrahydrofolate ligase activity
methenyltetrahydrofolate cyclohydrolase activity
methylenetetrahydrofolate dehydrogenase (NAD+) activity
methylenetetrahydrofolate dehydrogenase (NADP+) activity
protein binding
ATP binding
oxidoreductase activity
hydrolase activity
ligase activity
histidine biosynthetic process
neutrophil homeostasis
neural tube closure
purine nucleotide biosynthetic process
methionine metabolic process
one-carbon metabolic process
heart development
metabolic process
cellular amino acid biosynthetic process
serine family amino acid metabolic process
serine family amino acid biosynthetic process
methionine biosynthetic process
10-formyltetrahydrofolate biosynthetic process
folic acid-containing compound biosynthetic process
transsulfuration
tetrahydrofolate interconversion
oxidation-reduction process
somite development
purine ribonucleotide biosynthetic process
folic acid metabolic process
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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