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Invitrogen
MTHFD1L Polyclonal Antibody
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FIGURE: 1 / 4
MTHFD1L Antibody (PA5-100158) in ICC/IF
Immunocytochemistry analysis of MTHFD1L in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MTHFD1L polyclonal antibody (Product # PA5-100158) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MTHFD1L(Accession Q6UB35), corresponding to amino acid residues F942-F978.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MTHFD1L.
Target Information
One-carbon substituted forms of tetrahydrofolate (THF) are involved in the de novo synthesis of purines and thymidylate and support cellular methylation reactions through the regeneration of methionine from homocysteine. MTHFD1L is an enzyme involved in THF synthesis in mitochondria (Christensen et al., 2005 [PubMed 15611115]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10-formyl-THF synthetase; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Gene Aliases: 2410004L15Rik; AI647056; dJ292B18.2; FTHFSDC1; MTC1THFS; MTHFD1L
UniProt ID: (Human) Q9H789, (Mouse) Q3V3R1
Entrez Gene ID: (Human) 25902, (Rat) 361472, (Mouse) 270685
one-carbon metabolic process
folic acid-containing compound metabolic process
biological_process
folic acid-containing compound biosynthetic process
formate metabolic process
tetrahydrofolate metabolic process
oxidation-reduction process
10-formyltetrahydrofolate biosynthetic process
neural tube closure
tetrahydrofolate interconversion
folic acid metabolic process
embryonic neurocranium morphogenesis
embryonic viscerocranium morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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