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|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 30 and 304 of Human MTHFD2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-28169 targets MTHFD2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28169 immunogen is recombinant fragment corresponding to a region within amino acids 30 and 304 of Human MTHFD2.
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cardiac metabolic pathways affected in the mouse model of barth syndrome.
PA5-28169 was used in immunohistochemistry - frozen section and western blot to study the effect of Taz-knockdown on the mitochondrial processes in cardiac tissues
|Huang Y,Powers C,Madala SK,Greis KD,Haffey WD,Towbin JA,Purevjav E,Javadov S,Strauss AW,Khuchua Z||PloS one (10:null)||2015|
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase; Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; mitochondrial; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NMDMC