Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, *SK-N-DZ, *SK-N-SH.
Predicted reactivity: Mouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Protein Aliases: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; Methenyltetrahydrofolate cyclohydrolase; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NAD-dependent methylenetetrahydrofolate dehydrogenase
Gene Aliases: MTHFD2; NMDMC
UniProt ID: (Human) P13995
Entrez Gene ID: (Human) 10797