Invitrogen

MUNC18 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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MUNC18 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of MUNC18 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUNC18 Polyclonal Antibody (Product # PA5-30183) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Cytoplasmic and Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MUNC18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded U373 xenograft, using STXBP1 (Product # PA5-30183) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

MUNC18 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extract (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Rat Liver (Lane 3) and Rat Kidney (Lane 4). The blot was probed with Anti-MUNC18 Polyclonal Antibody (Product # PA5-30183, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to MUNC18 was observed in the Mouse and Rat brain, while it was not detected in Rat Liver and Kidney which are reported negative for MUNC18 expression. An additional band was observed at ~28kDa in mouse and Rat brain tissues.

MUNC18 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue lysates owing to their inherent genetic constitution. Relative expression of MUNC18 was observed in Mouse Brain and Rat Brain lysates in comparison to Mouse Liver and Mouse Kidney using product (Product # PA5-30183) in western blot. An additional band was observed at ~28kDa. {RE}

Product Details

PA5-30183

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:20,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 96 and 374 of Human STXBP1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.8 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547657

Product Specific Information

Recommended positive controls: A431, mouse brain, rat brain.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (91%), Chicken (97%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EIEE4; FLJ37475; hUNC18; MGC146482; minisatellite 10g detected by probe MMS10; munc-18c; MUNC18-1; N-Sec1; neuronal SEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; rbSec1; rop; RP11-56D16.3; Syntaxin-binding protein 1; UNC-18 homolog; unc-18-1; Unc-18A; Unc18-1

Gene Aliases: AI317162; AI326233; ANC18HA; MMS10-G; Ms10g; Munc-18a; MUNC18-1; N-sec1; NSEC1; NSEC1A; P67; Rb-sec1; RBSEC1; rbSec1A; rbSec1B; Sec1; STXBP1; Sxtbp1; UNC18; Unc18-1; UNC18A; Unc18h

UniProt ID: (Human) P61764, (Mouse) O08599, (Rat) P61765

Entrez Gene ID: (Human) 6812, (Mouse) 20910, (Rat) 25558

Molecular Function: membrane trafficking regulatory protein