Invitrogen

MUPP1 Polyclonal Antibody

Name: MUPP1 Polyclonal Antibody
Brand: Invitrogen
SKU: 42-2700

Advanced Verification

21 Published Figures

10 References

View all (11) MUPP1 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

MUPP1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 24

MUPP1 Antibody (42-2700) in IHC

Immunofluorescent staining of Schimdt-Lanterman incisures in fixed mouse sciatic nerve using Zymed Rb anti-MUPP1 (Product # 42-2700). Image courtesy of Dr. James Nagy, University of Manitoba, Winnipeg, Canada.

MUPP1 Antibody in Immunocytochemistry (ICC/IF)

MUPP1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

MUPP1 Antibody — Generation of global and conditional Mpdz knockout mice A Schematic representation of the Mpdz gene locus and the translated protein. The numbered boxes represent exons 1-47 of the wild-type allele. The relative position of the translational start and stop sites is indicated at exon 2 and exon 47, respectively. A gene-trap cassette (beta-geo) was inserted into intron 11-12 leading to a stop signal that truncates the Mpdz protein after the third PDZ domain. B Western blotting to detect Mpdz protein in brain lysates of littermate wild-type and global knockout Mpdz mice. C Schematic drawing of the conditional Mpdz gene targeting strategy. LoxP sites flanking exon 4 and a neomycin resistance cassette were inserted by homologous recombination. Transgenic mice were crossed with Flp deleter mice to remove the FRT-flanked neomycin cassette. Cre recombinase removes exons 4 and 5 leading to a frameshift and a nonsense mutation. This truncates the Mpdz protein after the L27 domain. D Western blotting to detect Mpdz protein in brain lysates of Mpdz fl/fl and CMV-Cre; Mpdz Delta/Delta mice. E, F Kaplan-Meier survival analysis of Mpdz -/- vs. Mpdz +/+ mice ( n > 41 mice per genotype) and CMV-Cre; Mpdz Delta/Delta vs. Mpdz fl/fl mice ( n > 48 mice per genotype). G, H Representative images of Mpdz -/- and Mpdz +/+ mice at postnatal day 27 (P27) and CMV-Cre; Mpdz Delta/Delta and Mpdz fl/fl mice at P24. Arrows indicate the enlarged and dome-shaped skull and the enlarged brain hemispheres. Sour {KO}

Immunogen image

Immunogen Image MUPP1 Polyclonal Antibody

Product Details

42-2700

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Gerbil, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of mouse MUPP1

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533517

Target Information

MUPP1 (multi PDZ domain protein 1, MPDZ) was discovered as an interacting partner with the 5-HT2C receptor1, and was found to co-localize with 5-HT2A or 5-HT2C receptors in all regions of the mouse brain, including the choroid plexus. MUPP1 contains 13 PDZ domains but no apparent catalytic domain. MUPP1 is concentrated in tight junctions of polarized epithelial cells and binds to claudin-1 and junctional adhesion molecule (JAM). MUPP1 may therefore function as a multivalent scaffold protein that recruits various proteins to tight junctions. MUPP1 also interacts with claudin-8 and may play a role in tight junction barrier function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp781P216; FLJ25909; FLJ34626; FLJ90240; Multi-PDZ domain protein 1; Multiple PDZ domain protein

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Gene Aliases: AI225843; B930003D11Rik; Mpdz; MUPP1

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UniProt ID: (Mouse) Q8VBX6

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Entrez Gene ID: (Mouse) 17475

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUPP1 Polyclonal Antibody

MUPP1 Antibody (42-2700) in IHC

Product Details

42-2700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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