Invitrogen

MX1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (18) MX1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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MX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — MX1 Antobody detects MX1 protein at cytosol on MDA-MB-468 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded MDA-MB-468 xenograft. MX1 Antobody (Product # PA5-22149) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

MX1 Antibody in Western Blot (WB) — Western blot was performed using MX1 Polyclonal Antibody (Product # PA5-22149) and a 91 kDa band corresponding to MX1 was observed in cell lines treated with IFN alpha. Whole cell extracts (30 µg lysate) of Daudi (Lane 1), Daudi treated with IFN Alpha (10ng/mL, 16 hrs) (Lane 2), HeLa (Lane 3) and HeLa treated with IFN Alpha (10ng/mL, 16 hrs) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MX1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of MX1 using MX1 Polyclonal Antibody (Product # PA5-22149) shows increased expression of MX1 protein in HeLa cell line upon treatment with IFN Alpha. {TM}

Product Details

PA5-22149

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:10,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 276 of MX1 (Uniprot ID#P20591) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.06 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11156211

Product Specific Information

Recommended positive controls: NCI-H929, human MX1-transfected 293T.

Predicted reactivity: Mouse (83%), Dog (85%), Pig (83%), Sheep (80%), Rhesus Monkey (96%), Chimpanzee (99%), Bovine (84%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: IFI-78K; Interferon-induced GTP-binding protein Mx1; Interferon-induced GTP-binding protein Mx1, N-terminally processed; Interferon-induced protein p78; interferon-inducible protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX dynamin-like GTPase 1; Myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; Myxovirus resistance protein 1

Gene Aliases: IFI-78K; IFI78; MX; MX1; MxA

UniProt ID: (Human) P20591

Entrez Gene ID: (Human) 4599

Molecular Function: membrane traffic protein