Proteintech

MYCN Polyclonal Antibody

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MYCN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP ( MYCN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MYCN Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Product Details

10159-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYCN Fusion Protein Ag0193 (289-464 aa encoded by BC002712)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: SNTKAVTTF TITVRPKNAA LGPGRAQSSE LILKRCLPIH QQHNYAAPSP YVESEDAPPQ KKIKSEASPR PLKSVIPPKA KSLSPRNSDS EDSERRRNHN ILERQRRNDL RSSFLTLRDH VPELVKNEKA AKVVILKKAT EYVHSLQAEE HQLLLEKEKL QARQQQLLKK IEHARTC (289-464 aa encoded by BC002712 )

Target Information

N-Myc protein is member of the MYC family of transcription factor proteins with a basic helix-loop-helix (bHLH) domain. N-Myc is similarly regulated and functionally complementary to C-Myc in the context of various cellular growth and differentiation processes. MYCN gene is considered the hallmark of neuroblastoma, amplification and overexpression of the MYCN proto-oncogene occurs in approximately 20% of neuroblastomas and is associated with advanced stage disease, rapid tumor progression, and poor prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low expression of MYCN promotes cisplatin resistance by suppressing cisplatin-induced apoptosis in epithelial ovarian cancer.

Oncology letters

Yu R,Zhang H,Wang R,Xiao L

Thu Dec 01 00:00:00 EST 2022

Low expression of MYCN promotes cisplatin resistance by suppressing cisplatin-induced apoptosis in epithelial ovarian cancer.

Oncology letters

Yu R,Zhang H,Wang R,Xiao L

Thu Dec 01 00:00:00 EST 2022

Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients.

Cancer research communications

Jung M,Kowalczyk K,Hankins R,Bandi G,Kallakury B,Carrasquilla MA,Banerjee PP,Grindrod S,Dritschilo A

Thu Dec 01 00:00:00 EST 2022

Clinically relevant treatment of PDX models reveals patterns of neuroblastoma chemoresistance.

Science advances

Mañas A,Aaltonen K,Andersson N,Hansson K,Adamska A,Seger A,Yasui H,van den Bos H,Radke K,Esfandyari J,Bhave MS,Karlsson J,Spierings D,Foijer F,Gisselsson D,Bexell D

Fri Oct 28 00:00:00 EDT 2022

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Nature

Burda JE,O'Shea TM,Ao Y,Suresh KB,Wang S,Bernstein AM,Chandra A,Deverasetty S,Kawaguchi R,Kim JH,McCallum S,Rogers A,Wahane S,Sofroniew MV

Wed Jun 01 00:00:00 EDT 2022

An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.

Frontiers in cell and developmental biology

Fan Z,Kong M,Miao X,Guo Y,Ren H,Wang J,Wang S,Tang N,Shang L,Zhu Z,Liu H,Zhu W,Shi X

Wed Nov 10 00:00:00 EST 2021

An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.

Frontiers in cell and developmental biology

Fan Z,Kong M,Miao X,Guo Y,Ren H,Wang J,Wang S,Tang N,Shang L,Zhu Z,Liu H,Zhu W,Shi X

Wed Nov 10 00:00:00 EST 2021

Comparative proteomics reveals Cryptosporidium parvum manipulation of the host cell molecular expression and immune response.

PLoS neglected tropical diseases

Li T,Liu H,Jiang N,Wang Y,Wang Y,Zhang J,Shen Y,Cao J

Mon Nov 01 00:00:00 EDT 2021

A Primer for Assessing the Pathology in Mouse Models of Neuroblastoma.

Current protocols

Gifford AJ,Murray J,Fletcher JI,Marshall GM,Norris MD,Haber M

Mon Nov 01 00:00:00 EDT 2021

Transcriptomic Analyses of MYCN-Regulated Genes in Anaplastic Wilms' Tumour Cell Lines Reveals Oncogenic Pathways and Potential Therapeutic Vulnerabilities.

Cancers

Szemes M,Melegh Z,Bellamy J,Park JH,Chen B,Greenhough A,Catchpoole D,Malik K

Sat Feb 06 00:00:00 EST 2021

Transcriptomic Analyses of MYCN-Regulated Genes in Anaplastic Wilms' Tumour Cell Lines Reveals Oncogenic Pathways and Potential Therapeutic Vulnerabilities.

Cancers

Szemes M,Melegh Z,Bellamy J,Park JH,Chen B,Greenhough A,Catchpoole D,Malik K

Sat Feb 06 00:00:00 EST 2021

Metabolic Evaluation of MYCN-Amplified Neuroblastoma by 4-[¹⁸F]FGln PET Imaging.

Molecular imaging and biology

Li C,Huang S,Guo J,Wang C,Huang Z,Huang R,Liu L,Liang S,Wang H

Sun Dec 01 00:00:00 EST 2019

Downregulation of PRMT1 promotes the senescence and migration of a non-MYCN amplified neuroblastoma SK-N-SH cells.

Scientific reports

Lee YJ,Chang WW,Chang CP,Liu TY,Chuang CY,Qian K,Zheng YG,Li C

Mon Feb 11 00:00:00 EST 2019

N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.

Molecular cancer

Yin Y,Xu L,Chang Y,Zeng T,Chen X,Wang A,Groth J,Foo WC,Liang C,Hu H,Huang J

Fri Jan 18 00:00:00 EST 2019

N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.

Molecular cancer

Yin Y,Xu L,Chang Y,Zeng T,Chen X,Wang A,Groth J,Foo WC,Liang C,Hu H,Huang J

Fri Jan 18 00:00:00 EST 2019

Histone Citrullination Represses MicroRNA Expression, Resulting in Increased Oncogene mRNAs in Somatolactotrope Cells.

Molecular and cellular biology

DeVore SB,Young CH,Li G,Sundararajan A,Ramaraj T,Mudge J,Schilkey F,Muth A,Thompson PR,Cherrington BD

Mon Oct 01 00:00:00 EDT 2018

Combined ALK and MDM2 inhibition increases antitumor activity and overcomes resistance in human ALK mutant neuroblastoma cell lines and xenograft models.

eLife

Wang HQ,Halilovic E,Li X,Liang J,Cao Y,Rakiec DP,Ruddy DA,Jeay S,Wuerthner JU,Timple N,Kasibhatla S,Li N,Williams JA,Sellers WR,Huang A,Li F

Thu Apr 20 00:00:00 EDT 2017

Combined ALK and MDM2 inhibition increases antitumor activity and overcomes resistance in human ALK mutant neuroblastoma cell lines and xenograft models.

eLife

Wang HQ,Halilovic E,Li X,Liang J,Cao Y,Rakiec DP,Ruddy DA,Jeay S,Wuerthner JU,Timple N,Kasibhatla S,Li N,Williams JA,Sellers WR,Huang A,Li F

Thu Apr 20 00:00:00 EDT 2017

Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer.

Frontiers in oncology

Cage TA,Chanthery Y,Chesler L,Grimmer M,Knight Z,Shokat K,Weiss WA,Gustafson WC

Mon Jun 01 00:00:00 EDT 2015

Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer.

Frontiers in oncology

Cage TA,Chanthery Y,Chesler L,Grimmer M,Knight Z,Shokat K,Weiss WA,Gustafson WC

Mon Jun 01 00:00:00 EDT 2015

Neuroblastoma of undifferentiated subtype, prognostic significance of prominent nucleolar formation, and MYC/MYCN protein expression: a report from the Children's Oncology Group.

Cancer

Wang LL,Suganuma R,Ikegaki N,Tang X,Naranjo A,McGrady P,London WB,Hogarty MD,Gastier-Foster JM,Look AT,Park JR,Maris JM,Cohn SL,Seeger RC,Shimada H

Tue Oct 15 00:00:00 EDT 2013

Neuroblastoma of undifferentiated subtype, prognostic significance of prominent nucleolar formation, and MYC/MYCN protein expression: a report from the Children's Oncology Group.

Cancer

Wang LL,Suganuma R,Ikegaki N,Tang X,Naranjo A,McGrady P,London WB,Hogarty MD,Gastier-Foster JM,Look AT,Park JR,Maris JM,Cohn SL,Seeger RC,Shimada H

Tue Oct 15 00:00:00 EDT 2013

Peripheral neuroblastic tumors with genotype-phenotype discordance: a report from the Children's Oncology Group and the International Neuroblastoma Pathology Committee.

Pediatric blood & cancer

Suganuma R,Wang LL,Sano H,Naranjo A,London WB,Seeger RC,Hogarty MD,Gastier-Foster JM,Look AT,Park JR,Maris JM,Cohn SL,Amann G,Beiske K,Cullinane CJ,d'Amore ES,Gambini C,Jarzembowski JA,Joshi VV,Navarro S,Peuchmaur M,Shimada H

Fri Mar 01 00:00:00 EST 2013

Peripheral neuroblastic tumors with genotype-phenotype discordance: a report from the Children's Oncology Group and the International Neuroblastoma Pathology Committee.

Pediatric blood & cancer

Fri Mar 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: Avian myelocytomatosis viral (v-myc) related oncogene neuroblastoma derived (Nmyc); Avian myelocytomatosis viral (v-myc) related oncogene, neuroblastoma derived (Nmyc); bHLHe37; Class E basic helix-loop-helix protein 37; N-myc proto-oncogene protein; neuroblastoma MYC oncogene; neuroblastoma myc-related oncogene 1; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; oncogene NMYC; oncogene pp65/67; pp65/67; v-myc myelocytomatosis viral related oncogene, neuroblastoma derived

Gene Aliases: BHLHE37; c-nmyc; MODED; MYCN; N-myc; Nmuc1; NMYC; Nmyc-1; Nmyc1; ODED

UniProt ID: (Human) P04198, (Mouse) P03966, (Rat) Q63379

Entrez Gene ID: (Human) 4613, (Mouse) 18109, (Rat) 298894

Molecular Function: basic helix-loop-helix transcription factor