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Invitrogen
MYF5 Polyclonal Antibody
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FIGURE: 1 / 7
MYF5 Antibody (PA5-115608) in ICC/IF
Immunocytochemistry analysis of MYF5 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115608) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115608
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYF5(Accession P13349), corresponding to amino acid residues E25-C75.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MYF5.
Target Information
Myogenic factor 5 (MYF5) is a gene located on chromosome 12q21.31, encoding a basic helix-loop-helix (bHLH) transcription factor essential for skeletal muscle development and differentiation. MYF5 is one of the key members of the myogenic regulatory factor (MRF) family, which also includes MyoD, myogenin, and MRF4. It plays a crucial role in myogenesis by regulating the expression of genes necessary for muscle precursor cell proliferation and differentiation into mature muscle fibers. During embryonic development, MYF5 is involved in the formation of muscle tissue and maintenance of muscle progenitor cells. Mutations or dysregulation in MYF5 can lead to developmental abnormalities in muscle formation and are implicated in muscle-related disorders. Research into MYF5 continues to explore its regulatory mechanisms in muscle biology and potential therapeutic applications in muscular dystrophies and muscle repair processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHc2; Class C basic helix-loop-helix protein 2; Myf-5; Myogenic factor 5; myogenic regulatory factor MYF-5
Gene Aliases: B130010J22Rik; BHLHC2; Myf-5; MYF5
UniProt ID: (Human) P13349, (Mouse) P24699
Entrez Gene ID: (Human) 4617, (Rat) 299766, (Mouse) 17877
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein heterodimerization activity
E-box binding
sequence-specific DNA binding
protein dimerization activity
DNA binding
basic helix-loop-helix transcription factor
cartilage condensation
ossification
somitogenesis
regulation of cell-matrix adhesion
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
muscle organ development
skeletal muscle tissue development
extracellular matrix organization
skeletal muscle cell differentiation
muscle cell fate commitment
camera-type eye development
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
embryonic skeletal system morphogenesis
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
muscle tissue morphogenesis
positive regulation of myoblast fusion
muscle organ morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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