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Invitrogen
MYF5 Polyclonal Antibody
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FIGURE: 1 / 1
MYF5 Antibody (PA5-79715) in WB
Western blot analysis of MYF5 in Lane 1: human SW620 cell lysate, Lane 2: mouse testis tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with MYF5 polyclonal antibody (Product # PA5-79715) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat ... View More
Product Details
PA5-79715
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Myf5 recombinant protein (Position: E25-Y146).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SW620 cell, mouse testis tissue.
Target Information
Myogenic factor 5 (MYF5) is a gene located on chromosome 12q21.31, encoding a basic helix-loop-helix (bHLH) transcription factor essential for skeletal muscle development and differentiation. MYF5 is one of the key members of the myogenic regulatory factor (MRF) family, which also includes MyoD, myogenin, and MRF4. It plays a crucial role in myogenesis by regulating the expression of genes necessary for muscle precursor cell proliferation and differentiation into mature muscle fibers. During embryonic development, MYF5 is involved in the formation of muscle tissue and maintenance of muscle progenitor cells. Mutations or dysregulation in MYF5 can lead to developmental abnormalities in muscle formation and are implicated in muscle-related disorders. Research into MYF5 continues to explore its regulatory mechanisms in muscle biology and potential therapeutic applications in muscular dystrophies and muscle repair processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHc2; Class C basic helix-loop-helix protein 2; Myf-5; Myogenic factor 5; myogenic regulatory factor MYF-5; unnamed protein product
Gene Aliases: B130010J22Rik; BHLHC2; EORVA; Myf-5; MYF5
UniProt ID: (Human) P13349, (Mouse) P24699
Entrez Gene ID: (Human) 4617, (Rat) 299766, (Mouse) 17877
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
sequence-specific DNA binding
protein dimerization activity
E-box binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
basic helix-loop-helix transcription factor
cartilage condensation
ossification
somitogenesis
regulation of cell-matrix adhesion
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
muscle organ development
skeletal muscle tissue development
tissue development
cell differentiation
extracellular matrix organization
skeletal muscle cell differentiation
muscle cell fate commitment
camera-type eye development
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
animal organ development
muscle organ morphogenesis
embryonic skeletal system morphogenesis
positive regulation of skeletal muscle fiber development
muscle tissue morphogenesis
transcription from RNA polymerase II promoter
positive regulation of myoblast fusion
transcription, DNA-templated
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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