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MYF6 Antibody (PA5-120339) in WB
Western blot analysis of MYF6 in 293T-MYF6 cells. Samples were incubated with MYF6 Polyclonal antibody (Product # PA5-120339) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120339
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-180 of human MYF6 (NP_002460.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
2.02 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913911
Product Specific Information
Positive test controls include: HepG2, Mouse skeletal muscle, Mouse spleen, Rat skeletal muscle. The target is usually found in the following locations: Nucleus.
Immunogen sequence: WACKTCKRKS APTDRRKAAT LRERRRLKKI NEAFEALKRR TVANPNQRLP KVEILRSAIS YIERLQDLLH RLDQQEKMQE LGVDPFSYRP KQENLEGADF L
Target Information
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHc4; Class C basic helix-loop-helix protein 4; Herculin; Muscle-specific regulatory factor 4; Myf-6; Myogenic factor 6; Myogenic factor 6 (herculin); myogenic factor 6 (herculin); myogenic factor-6
Gene Aliases: BHLHC4; CNM3; herculin; MRF4; MRF4AA; Myf-6; MYF6
UniProt ID: (Human) P23409, (Rat) P19335, (Mouse) P15375
Entrez Gene ID: (Human) 4618, (Rat) 25714, (Mouse) 17878
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein heterodimerization activity
E-box binding
molecular_function
protein dimerization activity
DNA binding
basic helix-loop-helix transcription factor
somitogenesis
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
skeletal muscle tissue development
skeletal muscle cell differentiation
muscle cell fate commitment
skeletal muscle tissue regeneration
positive regulation of myoblast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
muscle tissue morphogenesis
positive regulation of myoblast fusion
positive regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
muscle organ development
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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