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MYH10 Antibody (PA5-78065) in ICC/IF
MYH10 Polyclonal Antibody detects MYH10 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH10 protein stained by MYH10 Polyclonal Antibody (Product # PA5-78065) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human MYH10. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736179
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2, MYH10-transfected 293T
Predicted Reactivity: Mouse (100%), Rat (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MYH10 encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; myosin heavy chain, nonmuscle type B; myosin, heavy polypeptide 10, non-muscle; Myosin-10; NMMHC II-b; NMMHC IIB; NMMHC-B; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; nonmuscle myosin; nonmuscle myosin heavy chain IIB; nonmuscle myosin II heavy chain-B; OTTHUMP00000165799
Gene Aliases: MYH10; NMMHC-IIB; NMMHCB
UniProt ID: (Human) P35580
Entrez Gene ID: (Human) 4628
mitotic cytokinesis
in utero embryonic development
neuron migration
plasma membrane repair
cardiac septum development
exocytosis
substrate-dependent cell migration, cell extension
nuclear migration
cell adhesion
axon guidance
adult heart development
cell proliferation
regulation of cell shape
fourth ventricle development
lateral ventricle development
third ventricle development
cerebellar Purkinje cell layer development
actin filament-based movement
actomyosin structure organization
aorta development
neuromuscular process controlling balance
cardiac myofibril assembly
ventricular cardiac muscle cell development
retina development in camera-type eye
coronary vasculature development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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