Invitrogen

MYH14 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (11) MYH14 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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MYH14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MYH14 in human small intestine and lymph node tissues using MYH14 Polyclonal Antibody (Product # PA5-64345). Corresponding MYH14 RNA-seq data are presented for the same tissues.

MYH14 Antibody in Western Blot (WB) — Western blot analysis of MYH14 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a MYH14 Polyclonal Antibody (Product # PA5-64345).

MYH14 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MYH14 demonstrates antibody specificity. Immunohistochemical analysis of MYH14 using anti-MYH14 Polyclonal Antibody (Product # PA5-64345), shows significant staining of MYH14 in human small intestine and shows minimal or weak staining in human lymph node tissues. The relative expression levels of MYH14 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-64345

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human MYH14 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644340

Product Specific Information

Immunogen sequence: FTTRTVRQVF RLEEGVASDE EAEEAQPGSG PSPEPEGSPP

Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 80%.

Target Information

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MYH14 variant protein; Myosin heavy chain 14; Myosin heavy chain, non-muscle IIc; myosin, heavy polypeptide 14; Myosin-14; NMHC II-C; Non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C

Gene Aliases: DFNA4; DFNA4A; FP17425; KIAA2034; MHC16; MYH14; MYH17; myosin; NMHC II-C; NMHC-II-C; PNMHH

UniProt ID: (Human) Q7Z406

Entrez Gene ID: (Human) 79784

Function(s)

skeletal muscle contraction skeletal muscle tissue development sensory perception of sound regulation of cell shape neuronal action potential actin filament-based movement actomyosin structure organization mitochondrion morphogenesis vocalization behavior

Process(es)

microfilament motor activity calmodulin binding ATP binding actin-dependent ATPase activity actin filament binding