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MYH7 Antibody (PA5-100023) in ICC/IF
Immunofluorescent analysis of MYH7 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MYH7 polyclonal antibody (Product # PA5-100023) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100023
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MYH7(Accession P12883), corresponding to amino acid residues E1705-A1755.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815553
Product Specific Information
Antibody detects endogenous levels of total MYH7.
Target Information
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta myosin heavy chain; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain beta isoform; MYH-beta/slow; MyHC-beta; MyHC-slow; myopathy, distal 1; Myosin heavy chain 7; myosin heavy chain polypeptide 7 cardiac muscle fetal; Myosin heavy chain slow isoform; Myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 7; myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A
Gene Aliases: B-MHC; beta-MHC; CMD1S; CMH1; MPD1; MYH7; Myhc-b; MyHC-I; MYHCB; SPMD; SPMM
UniProt ID: (Human) P12883, (Mouse) Q91Z83, (Rat) P02564
Entrez Gene ID: (Human) 4625, (Mouse) 140781, (Rat) 29557
regulation of the force of heart contraction
regulation of heart rate
skeletal muscle contraction
muscle contraction
striated muscle contraction
adult heart development
regulation of the force of skeletal muscle contraction
transition between fast and slow fiber
muscle filament sliding
regulation of slow-twitch skeletal muscle fiber contraction
ATP metabolic process
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
metabolic process
response to reactive oxygen species
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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