Proteintech

MYH9 Polyclonal Antibody

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MYH9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 11128-1-AP (MYH9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MYH9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 11128-1-AP (MYH9 antibody) at dilution of 1:100 (under 40x lens).

MYH9 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MYH9 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human MYH9 (Product # 11128-1-AP) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) at dilution 1:200 (red), or 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .

MYH9 Antibody in Immunoprecipitation (IP) — IP result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

11128-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 11 publications 11 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYH9 Fusion Protein Ag1612 (1741-1960 aa encoded by BC011915)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: EQGNTELIN DRLKKANLQI DQINTDLNLE RSHAQKNENA RQQLERQNKE LKVKLQEMEG TVKSKYKASI TALEAKIAQL EEQLDNETKE RQAACKQVRR TEKKLKDVLL QVDDERRNAE QYKDQADKAS TRLKQLKRQL EEAEEEAQRA NASRRKLQRE LEDATETADA MNREVSSLKN KLRRGDLPFV VPRRMARKGA GDGSDEEVDG KADGAEAKPA E (1741-1960 aa encoded by BC011915)

Target Information

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomics.

Journal of neurochemistry

Xu H,Bensalel J,Raju S,Capobianco E,Lu ML,Wei J

Wed Feb 01 00:00:00 EST 2023

The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury induced by LPS via NMMHC IIA/Wnt5a/β-catenin pathway.

Toxicology and applied pharmacology

Zhang J,Pan Z,Zhou J,Zhang L,Tang J,Gong S,Li F,Yu B,Zhang Y,Kou J

Thu Sep 01 00:00:00 EDT 2022

The myosin II inhibitor, blebbistatin, ameliorates pulmonary endothelial barrier dysfunction in acute lung injury induced by LPS via NMMHC IIA/Wnt5a/β-catenin pathway.

Toxicology and applied pharmacology

Zhang J,Pan Z,Zhou J,Zhang L,Tang J,Gong S,Li F,Yu B,Zhang Y,Kou J

Thu Sep 01 00:00:00 EDT 2022

A functional role of S100A4/non-muscle myosin IIA axis for pro-tumorigenic vascular functions in glioblastoma.

Cell communication and signaling : CCS

Inukai M,Yokoi A,Ishizuka Y,Hashimura M,Matsumoto T,Oguri Y,Nakagawa M,Ishibashi Y,Ito T,Kumabe T,Saegusa M

Thu Apr 07 00:00:00 EDT 2022

A functional role of S100A4/non-muscle myosin IIA axis for pro-tumorigenic vascular functions in glioblastoma.

Cell communication and signaling : CCS

Inukai M,Yokoi A,Ishizuka Y,Hashimura M,Matsumoto T,Oguri Y,Nakagawa M,Ishibashi Y,Ito T,Kumabe T,Saegusa M

Thu Apr 07 00:00:00 EDT 2022

SRSF3 facilitates replication of influenza A virus via binding and promoting the transport of viral mRNA.

Veterinary microbiology

Zhao L,Li Y,Zhao Y,Liu Q,Lu Y,Ping J

Tue Mar 01 00:00:00 EST 2022

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling.

Cell death and differentiation

Hu S,Ren S,Cai Y,Liu J,Han Y,Zhao Y,Yang J,Zhou X,Wang X

Tue Mar 01 00:00:00 EST 2022

Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling.

Cell death and differentiation

Hu S,Ren S,Cai Y,Liu J,Han Y,Zhao Y,Yang J,Zhou X,Wang X

Tue Mar 01 00:00:00 EST 2022

YiQiFuMai lyophilized injection attenuates cerebral ischemic injury with inhibition of neuronal autophagy through intervention in the NMMHC IIA-actin-ATG9A interaction.