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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Dog, Human, Rat, Xenopus |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide, conjugated to KLH, corresponding to residues 1950-1961 of human non-muscle Myosin IIA. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
10.2 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
0.01M PBS, pH 7.4, with 1% BSA |
Contains |
15mM sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_795005 |
Recommended positive controls: MDCK, Jurkat, NRK.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cellular myosin heavy chain, type A; MGC104539; Myosin Heavy Chain 2A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; myosin IIA; myosin, heavy polypeptide 9, non-muscle; Myosin-9; myosin-9-like; myosin9; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; NMMHC-IIA; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A; nonmuscle myosin II heavy chain A
Gene Aliases: BDPLT6; DFNA17; EPSTS; FTNS; KLG/PTK7; MHA; MYH9; myosin-2a; NMHC-II-A; NMMHC-IIA; NMMHCA; XELAEV_18023533mg
UniProt ID: (Human) P35579, (Dog) Q258K2
Entrez Gene ID: (Rat) 100911597, (Human) 4627, (Dog) 481280, (Xenopus) 398083
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