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MYH9 Antibody (PA5-120506) in WB
Western blot analysis of MYH9 in extracts of various cell lines. Samples were incubated with MYH9 Polyclonal antibody (Product # PA5-120506) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1801-1960 of human MYH9 (NP_002464.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.79 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2914078
Product Specific Information
Positive test controls include: HeLa, HT-29, A-549, NIH/3T3, C6. The target is usually found in the following locations: Cytoplasm, cell cortex, cytoskeleton.
Target Information
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular myosin heavy chain, type A; flectin; MGC104539; Myosin heavy chain 9; myosin heavy chain IX; Myosin heavy chain, non-muscle IIa; myosin IIA; Myosin-9; myosin-9-like; myosin9; NMHC II-A; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Gene Aliases: BDPLT6; DFNA17; EPSTS; Fltn; FTNS; MHA; MYH9; Myhn-1; Myhn1; NMHC-II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; NMMHCA; TU72.6
UniProt ID: (Human) P35579, (Mouse) Q8VDD5
Entrez Gene ID: (Rat) 100911597, (Human) 4627, (Mouse) 17886
meiotic spindle organization
cell morphogenesis involved in differentiation
cytokinesis
angiogenesis
in utero embryonic development
establishment of T cell polarity
membrane protein ectodomain proteolysis
phagocytosis, engulfment
movement of cell or subcellular component
cell adhesion
myoblast fusion
regulation of cell shape
protein transport
single organismal cell-cell adhesion
actin filament polymerization
actin filament-based movement
platelet formation
actomyosin structure organization
actin cytoskeleton reorganization
uropod organization
blood vessel endothelial cell migration
establishment of meiotic spindle localization
actin filament capping
platelet aggregation
negative regulation of actin filament severing
positive regulation of protein processing in phagocytic vesicle
integrin-mediated signaling pathway
monocyte differentiation
leukocyte migration
metabolic process
microfilament motor activity
motor activity
actin binding
ATP binding
ATPase activity
actin-dependent ATPase activity
protein homodimerization activity
protein anchor
ADP binding
poly(A) RNA binding
actin filament binding
protein binding
calmodulin binding
protein domain specific binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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