Immunofluorescent analysis of Myosin IIa in HeLa cells using a Myosin IIa polyclonal antibody (Product # PA5-17025) (green) showing colocalization with actin filaments that have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Fungi|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with the nonmuscle heavy chains of myosin IIb or IIc.
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement.
PA5-17025 was used in immunocytochemistry to investigate how the cortical actin meshwork in the rear develops
|Ramalingam N,Franke C,Jaschinski E,Winterhoff M,Lu Y,Brühmann S,Junemann A,Meier H,Noegel AA,Weber I,Zhao H,Merkel R,Schleicher M,Faix J||Nature communications (6:null)||2015|