Invitrogen

MYH9 Polyclonal Antibody

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MYH9 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of MYH9 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: MYH9 Polyclonal Antibody (Product # PA5-29673) diluted at 1:500. Blue: Hoechst 33342 staining.

MYH9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of MYH9 was performed in paraffin-embedded human breast carcinoma tissue using MYH9 Polyclonal Antibody (Product # PA5-29673) at a dilution of 1:500.

MYH9 Antibody in Western Blot (WB) — Western blot was performed using Anti-MYH9 Polyclonal Antibody (Product # PA5-29673) and a 226kDa band corresponding to MYH9 was observed in Mouse Heart and was not present in Mouse Lung. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1) and Mouse Lung (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MYH9 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of MYH9 was performed in HeLa whole cell extracts using 5 µg of MYH9 Polyclonal Antibody (Product # PA5-29673). Samples were transferred to a membrane and probed with MYH9 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

MYH9 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 5 MiR-335-5p targeted MYH9. (a-c) The levels of MYH9 mRNA and protein in OS tissues were analyzed by RT-qPCR, western blotting, and IHC. (d) The level of MYH9 protein in OS cells was analyzed by western blotting. (e) The binding sites between MYH9 and miR-335-5p were predicted by the starBase database. (f and g) The luciferase activities of the reporter plasmids carrying MYH9 3'UTR-wt and MYH9 3'UTR-mut were determined with dual-luciferase reporter assay. (h and i) The abundance of miR-335-5p and MYH9 in the immunoprecipitates of the anti-Ago2 and anti-IgG groups was assessed by RT-qPCR. (j) The impacts of miR-335-5p overexpression and silencing on the level of MYH9 protein were analyzed by Western blotting. (k) The level of MYH9 protein in OS cells transfected with sh-NC + anti-NC, sh-circATP2A2 + anti-NC, or sh-circATP2A2 + anti-miR-335-5p was detected by Western blotting. * P < 0.05.

Product Details

PA5-29673

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 170 of Human MYH9

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547148

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, BCL-1, Raw264.7, C2C12, Rat2.

Predicted reactivity: Mouse (98%), Rat (97%), Xenopus laevis (91%), Dog (99%), Chicken (95%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

circATP2A2 promotes osteosarcoma progression by upregulating MYH9.

Open medicine (Warsaw, Poland)

Cao X,Meng X,Fu P,Wu L,Yang Z,Chen H

Published figure using MYH9 polyclonal antibody (Product # PA5-29673) in Western Blot

Wed Dec 15 00:00:00 EST 2021

circATP2A2 promotes osteosarcoma progression by upregulating MYH9.

Open medicine (Warsaw, Poland)

Cao X,Meng X,Fu P,Wu L,Yang Z,Chen H

Published figure using MYH9 polyclonal antibody (Product # PA5-29673) in Western Blot

Wed Dec 15 00:00:00 EST 2021

Bioinformatics

Protein Aliases: Cellular myosin heavy chain, type A; flectin; MGC104539; Myosin heavy chain 9; myosin heavy chain IX; Myosin heavy chain, non-muscle IIa; myosin IIA; Myosin-9; myosin-9-like; myosin9; NMHC II-A; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A

Gene Aliases: BDPLT6; DFNA17; EPSTS; Fltn; FTNS; MHA; MYH9; Myhn-1; Myhn1; NMHC-II-A; NMHCIIA; NMMHC-A; NMMHC-IIA; NMMHCA; TU72.6

UniProt ID: (Human) P35579, (Mouse) Q8VDD5

Entrez Gene ID: (Rat) 100911597, (Human) 4627, (Mouse) 17886

Function(s)