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Product Details
21642-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
MYLK Fusion Protein Ag16306 (1748-1914 aa encoded by BC113456)
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Smooth muscle MYLK generates three different proteins: long MYLK, short MYLK, and telokin. The 130 kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and this MYLK antibody can recognize these isoforms.
Immunogen sequence: TGNAVRAIG RLSSMAMISG LSGRKSSTGS PTSPLNAEKL ESEEDVSQAF LEAVAEEKPH VKPYFSKTIR DLEVVEGSAA RFDCKIEGYP DPEVVWFKDD QSIRESRHFQ IDYDEDGNCS LIISDVCGDD DAKYTCKAVN SLGEATCTAE LIVETMEEGE GEGEEEEE (1748-1914 aa encoded by BC113456)
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Kinase-related protein; KRP; MLCK; mylk; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin
Gene Aliases: 9530072E15Rik; A930019C19Rik; AAT7; AW489456; KRP; MLCK; MLCK1; MLCK108; MLCK210; MSTP083; MYLK; MYLK1; nmMlck; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638, (Mouse) 107589, (Rat) 288057
actin binding
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
metal ion binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
enzyme binding
positive regulation of defense response to virus by host
protein phosphorylation
smooth muscle contraction
actin filament organization
tonic smooth muscle contraction
phosphorylation
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
mitophagy in response to mitochondrial depolarization
muscle contraction
xenophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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