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Invitrogen
MYLK Polyclonal Antibody
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FIGURE: 1 / 10
MYLK Antibody (PA5-79716) in ICC/IF
Immunofluorescence analysis of MYLK was performed using 70% confluent log phase Caco-2 cells and Caco-2 cells treated with 10 ng/mL of IFN gamma for 18 hr followed by 2.5 ng/mL of TNF alpha for 4 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYLK Rabbit Polyclonal Antibody (Product # PA5-79716) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled wit... View More
Product Details
PA5-79716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MYLK recombinant protein (Position: D1441-D1709).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SGC-7901 whole cell, rat spleen tissue, mouse spleen tissue. IHC: mouse lung tissue, mouse small intestine tissue, rat small intestine tissue, human placenta tissue, human rectal cancer tissue, rat lung tissue.
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in the production of contractile activity (myosin-actin interaction). MLCK is responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Kinase-related protein; KRP; MLCK; mylk; Myosin light chain kinase, smooth muscle; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; Telokin; unnamed protein product
Gene Aliases: 9530072E15Rik; A930019C19Rik; AAT7; AW489456; KRP; MLCK; MLCK1; MLCK108; MLCK210; MMIHS; MMIHS1; MSTP083; MYLK; MYLK-L; MYLK1; nmMlck; smMLCK
UniProt ID: (Human) Q15746
Entrez Gene ID: (Human) 4638, (Mouse) 107589, (Rat) 288057
nucleotide binding
actin binding
protein kinase activity
protein serine/threonine kinase activity
myosin light chain kinase activity
protein binding
calmodulin binding
ATP binding
kinase activity
transferase activity
metal ion binding
scaffold protein binding
calmodulin-dependent protein kinase activity
transferase activity, transferring phosphorus-containing groups
enzyme binding
positive regulation of defense response to virus by host
protein phosphorylation
smooth muscle contraction
actin filament organization
tonic smooth muscle contraction
phosphorylation
positive regulation of cell migration
bleb assembly
positive regulation of calcium ion transport
aorta smooth muscle tissue morphogenesis
cellular hypotonic response
positive regulation of wound healing
mitophagy in response to mitochondrial depolarization
muscle organ development
regulation of synaptic vesicle endocytosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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