Invitrogen

MYLK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (11) MYLK2 antibodies

Questions & Answers

Questions & Answers

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MYLK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of MYLK2 in human cell line U-2 OS using a MYLK2 Polyclonal Antibody (Product # PA5-63768) shows localization to endoplasmic reticulum.

MYLK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MYLK2 in human skeletal muscle and heart muscle tissues using MYLK2 Polyclonal Antibody (Product # PA5-63768). Corresponding MYLK2 RNA-seq data are presented for the same tissues.

MYLK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MYLK2 in human skeletal muscle shows moderate cytoplasmic positivity in myocytes. Samples were probed using a MYLK2 Polyclonal Antibody (Product # PA5-63768).

MYLK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of MYLK2 in human heart muscle using MYLK2 Polyclonal Antibody (Product # PA5-63768) shows low expression as expected.

MYLK2 Antibody in Western Blot (WB) — Western blot analysis of MYLK2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MYLK2 Polyclonal Antibody (Product # PA5-63768).

MYLK2 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MYLK2 demonstrates antibody specificity. Immunohistochemical analysis of MYLK2 using anti-MYLK2 Polyclonal Antibody (Product # PA5-63768), shows significant staining of MYLK2 in human skeletal muscle and shows minimal or weak staining in human heart muscle tissues. The relative expression levels of MYLK2 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-63768

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human MYLK2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644352

Product Specific Information

Immunogen sequence: IEFQAVPSEK SEVGQALCLT AREEDCFQIL DDCPPPPAPF PHRMVELRTG NVSSEFSMNS KEALGGGKFG AVCTCMEKAT

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 89%.

Target Information

This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MLCK2; myosin light chain kinase 2, skeletal muscle; Myosin light chain kinase 2, skeletal/cardiac muscle

Gene Aliases: KMLC; MLCK; MLCK2; MYLK2; skMLCK

UniProt ID: (Human) Q9H1R3

Entrez Gene ID: (Human) 85366

Molecular Function: non-receptor serine/threonine protein kinase

striated muscle contraction neuromuscular synaptic transmission positive regulation of gene expression skeletal muscle satellite cell differentiation peptidyl-threonine phosphorylation regulation of muscle filament sliding skeletal muscle cell differentiation protein autophosphorylation cardiac muscle tissue morphogenesis cardiac muscle contraction

Process(es)

calmodulin-dependent protein kinase activity myosin light chain kinase activity protein binding calmodulin binding ATP binding