Immunofluorescence analysis of MYO7A was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYO7A Rabbit Polyclonal Antibody (Product # PA1-936) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

MYO7A Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

MYO7A Antibody in Immunohistochemistry (IHC)

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MYO7A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of MYO7A was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Bone and Rat Heart in Western Blot using MYO7A Polyclonal Antibody (Product # PA1-936). MYO7A is reported to be expressed in skeletal tissues and not in other tissues like bone and heart. {RE}

Immunogen image

Immunogen Image MYO7A Polyclonal Antibody

Product Details

PA1-936

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

200 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2235704

Product Specific Information

PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.

PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.

PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.

Target Information

Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: motor protein; MyO VIIa; myosin 7a; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; Unconventional myosin-VIIa

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Gene Aliases: Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B

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UniProt ID: (Mouse) P97479

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Entrez Gene ID: (Mouse) 17921, (Rat) 266714

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Function(s)

microfilament motor activity nucleotide binding motor activity actin binding protein binding calmodulin binding ATP binding protein domain specific binding spectrin binding actin-dependent ATPase activity protein complex binding protein homodimerization activity ADP binding protein N-terminus binding actin filament binding actin binding motor protein

Process(es)

phagolysosome assembly transport intracellular protein transport phagocytosis lysosome organization sensory perception visual perception sensory perception of sound metabolic process cell projection organization actin filament-based movement inner ear morphogenesis mechanoreceptor differentiation auditory receptor cell differentiation post-embryonic organ morphogenesis inner ear development sensory perception of light stimulus equilibrioception pigment granule localization pigment granule transport auditory receptor cell stereocilium organization inner ear receptor cell differentiation inner ear receptor stereocilium organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYO7A Polyclonal Antibody

MYO7A Antibody (PA1-936) in ICC/IF

Product Details

PA1-936

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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