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Invitrogen
MYO7A Polyclonal Antibody
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FIGURE: 1 / 13
MYO7A Antibody (PA1-936) in ICC/IF
Immunofluorescence analysis of MYO7A was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYO7A Rabbit Polyclonal Antibody (Product # PA1-936) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More
Product Details
PA1-936
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
C. elegans,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.
PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.
PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.
Target Information
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: motor protein; MyO VIIa; myosin 7a; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; Unconventional myosin-VIIa
Gene Aliases: Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B
UniProt ID: (Mouse) P97479
Entrez Gene ID: (Mouse) 17921, (Rat) 266714
microfilament motor activity
nucleotide binding
motor activity
actin binding
protein binding
calmodulin binding
ATP binding
protein domain specific binding
spectrin binding
actin-dependent ATPase activity
protein complex binding
protein homodimerization activity
ADP binding
protein N-terminus binding
actin filament binding
actin binding motor protein
phagolysosome assembly
transport
intracellular protein transport
phagocytosis
lysosome organization
sensory perception
visual perception
sensory perception of sound
metabolic process
cell projection organization
actin filament-based movement
inner ear morphogenesis
mechanoreceptor differentiation
auditory receptor cell differentiation
post-embryonic organ morphogenesis
inner ear development
sensory perception of light stimulus
equilibrioception
pigment granule localization
pigment granule transport
auditory receptor cell stereocilium organization
inner ear receptor cell differentiation
inner ear receptor stereocilium organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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